Quantifying the pathology of neurodegenerative disorders: quantitative measurements, sampling strategies and data analysis

Histopathology
Richard A Armstrong

Abstract

The use of quantitative methods has become increasingly important in the study of neurodegenerative disease. Disorders such as Alzheimer's disease (AD) are characterized by the formation of discrete, microscopic, pathological lesions which play an important role in pathological diagnosis. This article reviews the advantages and limitations of the different methods of quantifying the abundance of pathological lesions in histological sections, including estimates of density, frequency, coverage, and the use of semiquantitative scores. The major sampling methods by which these quantitative measures can be obtained from histological sections, including plot or quadrat sampling, transect sampling, and point-quarter sampling, are also described. In addition, the data analysis methods commonly used to analyse quantitative data in neuropathology, including analyses of variance (anova) and principal components analysis (PCA), are discussed. These methods are illustrated with reference to particular problems in the pathological diagnosis of AD and dementia with Lewy bodies (DLB).

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Citations

Sep 22, 2009·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Richard A Armstrong, Nigel J Cairns
Jan 1, 2004·Neuroscience Letters·N J CairnsR A Armstrong
Jul 25, 2007·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Richard A ArmstrongPeter L Lantos
Sep 3, 2013·Journal of Neural Transmission·Richard A ArmstrongNigel J Cairns
Oct 23, 2016·Journal of Neural Transmission·Richard A ArmstrongNigel J Cairns
Nov 6, 2015·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·David J IrwinJohn Q Trojanowski
Jan 15, 2004·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Richard A Armstrong
Sep 25, 2014·Journal of Cellular Physiology·Sandra A AcostaCesar V Borlongan
Jul 30, 2015·Stroke; a Journal of Cerebral Circulation·Sandra A AcostaCesar V Borlongan
Aug 27, 2009·Clinical Orthopaedics and Related Research·Christian J ZainoJoseph F Fetto
Dec 12, 2017·Brain : a Journal of Neurology·David J IrwinMurray Grossman
Sep 7, 2020·Neuropathology and Applied Neurobiology·D G CoughlinD J Irwin
Feb 25, 2021·Acta Neuropathologica Communications·Lucia A A GianniniDavid J Irwin

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