Quantifying vesicle/mixed micelle partitioning of phosphatidylcholine in model bile by using radiolabeled phosphatidylcholine species

The Journal of Laboratory and Clinical Medicine
W RenooijG P van Berge Henegouwen

Abstract

Phospholipid in vesicles and mixed micelles of (model) bile has been traced or quantitated (or both) by adding radioactively labeled phosphatidylcholine species. The question is whether these labeled species mix homogeneously with the phosphatidylcholine species mixture present, such that the label distribution reflects the already established mass partitioning of species. In this study, model bile containing egg yolk phosphatidylcholine was incubated with radioactive phosphatidylcholine species. Vesicle and mixed micelle fractions were separated by gel filtration. Radiochemical analysis of the species distribution confirmed chemical analysis: 1,2-di(14C)palmitoyl-phosphatidylcholine was enriched in the vesicles, the 1-palmitoyl-2-(14C)oleoyl species evenly distributed, and the 1-palmitoyl-2-(14C)linoleoyl species more expressed in mixed micelles. This indicates that the distribution of an added radioactive phosphatidylcholine species represents the vesicle/mixed micelle distribution of that particular phosphatidylcholine species. Consequently, the label distribution of a particular added radioactive phosphatidylcholine species can be used to calculate the vesicle/mixed micelle partitioning of total phosphatidylcholine only aft...Continue Reading

References

Jul 22, 1975·Biochimica Et Biophysica Acta·V OldenborgL M Van Golde
Apr 1, 1989·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C E Schriever, D Jüngst
Jan 19, 1988·Biochimica Et Biophysica Acta·P R HarveyS M Strasberg
Oct 24, 1986·Biochimica Et Biophysica Acta·G J SömjenT Gilat
Nov 1, 1986·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R T Holzbach
Feb 25, 1985·FEBS Letters·N R Pattinson
Jun 13, 1983·FEBS Letters·G J Somjen, T Gilat
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

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