Quantitating distance-dependent, indirect cell-cell interactions with a multilayered phospholipid polymer hydrogel

Biomaterials
Botao GaoKazuhiko Ishihara

Abstract

Multilayered polymer hydrogels containing living cells were assembled for assessing the distance-dependent effects of soluble factors secreted by stroma cells on tumor cell cycle progression in vitro. A layer of tumor cells and a layer of stroma cells were separated with finely controlled spacing in a multilayered sandwich composed of a 2-methacryloyloxyethyl phosphorylcholine polymer and poly(vinyl alcohol) hydrogel. We demonstrated the utility of this tool for investigating intercellular communication between human cervical cancer HeLa cells and supportive stromal L929 fibroblast cells in co-culture. Time-lapse microscopic analyses of HeLa cells showed short distances (15 μm) between tumor cells and stroma cells induced a continuous increase in the percentage of HeLa cells in the S/G2/M phases of the cell cycle, while longer distances (70 μm) between the cells caused a slower increase followed by a sharp increase in the percentage of cells in S/G2/M phases. One possible explanation is gradient formation in the diffusion-dominant multilayer hydrogels by water-soluble factors such as those inducing growth, differentiation, and proliferation. This study provides insights into the potential effects of diffusion of soluble factors...Continue Reading

References

Feb 13, 2008·Cell·Asako Sakaue-SawanoAtsushi Miyawaki
Jan 9, 2010·Trends in Biotechnology·Dietmar W HutmacherJudith A Clements
Dec 20, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Tatsuo AikawaKazuhiko Ishihara
Jun 19, 2012·Biosensors & Bioelectronics·Surasak Chantasirichot, Kazuhiko Ishihara
Aug 8, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ioannis K ZervantonakisRoger D Kamm
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Oct 31, 2012·Colloids and Surfaces. B, Biointerfaces·Xiaojie LinKazuhiko Ishihara
Apr 6, 2013·Biomedical Microdevices·Peter DelNeroClaudia Fischbach
Apr 17, 2013·Colloids and Surfaces. B, Biointerfaces·Botao GaoKazuhiko Ishihara
May 7, 2013·Annual Review of Biomedical Engineering·David W InfangerClaudia Fischbach

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Citations

May 7, 2021·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Tomohiro Konno
Mar 28, 2018·ACS Applied Materials & Interfaces·Liliang OuyangWei Sun

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