PMID: 6973602Aug 1, 1981Paper

Quantitation and clonal isolation of cytolytic T lymphocyte precursors selectively infiltrating murine sarcoma virus-induced tumors

The Journal of Experimental Medicine
K T BrunnerJ C Cerottini

Abstract

A limiting dilution mixed leukocyte-tumor cell microculture system was used to quantitate cytolytic T lymphocytes and their precursors (CTL-P), which infiltrate tumors induced by injection of Moloney sarcoma-leukemia virus (MSV-MoLV) complex into C57BL/6 mice. Leukocyte populations obtained from tumors collected on day 10 after virus injection were found to contain significantly higher frequencies of operationally defined (tumor-specific) CTL-P than either peripheral blood leukocytes (PBL) or spleen cells from the same animals. When these frequencies were normalized according to the content of Lyt-2+ T cells in each tissue, average CTL-P frequencies were found to be 1/9 in tumor-infiltrating cells vs. 1/41 in PBL. These results directly demonstrate selective accumulation of CTL-P in the tumor mass. A number of clonal isolates obtained from tumor-infiltrating leukocyte populations were expanded and studied for cytolytic activity and specificity. Of 11 isolates, 10 were found to have high cytolytic activity, leading to 50% lysis of the syngeneic MoLV-derived tumor target cells in 3.5 h at lymphocyte:target cell ratios ranging from 0.5:1 to 3.2:1. Furthermore, five randomly selected clones showed H-2 restriction by their selective...Continue Reading

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Citations

Jan 1, 1983·Cancer Immunology, Immunotherapy : CII·J BubeníkJ Neuwirt
Jul 17, 1990·Klinische Wochenschrift·J LorenzR Ferlinz
Jan 1, 1995·Immunologic Research·H Fujiwara, T Hamaoka
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M O DaileyI L Weissman
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·N UtsunomiyaM Nakanishi
Oct 1, 1982·The Journal of Experimental Medicine·H D EngersG D Sorenson
Apr 28, 2018·Nature Reviews. Cancer·Shelly Maman, Isaac P Witz
Mar 7, 2002·International Reviews of Immunology·P H BasseR B Herberman
Jan 1, 1997·International Journal of Clinical & Laboratory Research·B J Van den Eynde, T Boon
Oct 15, 1983·International Journal of Cancer. Journal International Du Cancer·H BinzH Wigzell

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