PMID: 6135411Apr 1, 1983

Quantitation by ELISA of pili and sheep antibodies to the pili of Bacteroides nodosus

Australian Veterinary Journal
K J FaheyB L Clark

Abstract

ELISA systems have been developed to quantitate the isotypic antibody response of sheep naturally infected with B. nodosus isolate 198 or injected with pili from isolate 198 in oil emulsion vaccines. The predominant humoral antibody detected following vaccination was IgG1, with substantially lower amounts of IgG2 and IgM. The antibody response was relatively specific for the pilus antigen from isolate 198. Although weak cross reactivities were detected with antiserums to some other isolates, ELISA IgG antibody titres in excess of 200 offer a tentative identification of the isolates of B. nodosus involved in natural outbreaks of footrot. A related ELISA was also developed to quantitate the amount of pili in cell suspensions and crude preparations of pili used in vaccines.

References

Mar 1, 1975·Journal of General Microbiology·T M Skerman
Jan 1, 1973·Journal of Comparative Pathology·J R Egerton
Feb 1, 1980·Infection and Immunity·T K KorhonenC S Edén
Jan 1, 1980·Methods in Enzymology·B A Hurn, S M Chantler
Jan 1, 1981·Journal of Immunological Methods·D G OliverJ W Hellman
Jun 30, 1956·Acta Medica Scandinavica·J HOREJSI, R SMETANA

Citations

Apr 1, 1994·Vaccine·J D HuntD J Stewart
Apr 1, 1984·Veterinary Microbiology·D L Emery, D J Stewart
Jan 12, 1993·Journal of Animal Breeding and Genetics = Zeitschrift Für Tierzüchtung Und Züchtungsbiologie·H W RaadsmaS C Brown
Aug 8, 2007·Veterinary Microbiology·Tulay I Cagatay, Jon G H Hickford
Apr 1, 1986·Australian Veterinary Journal·D J StewartI J O'Donnell
Apr 1, 1985·Australian Veterinary Journal·D J StewartR G Jarrett
Mar 1, 1984·Australian Veterinary Journal·D L EmeryB L Clark
Oct 20, 2005·Human Fertility : Journal of the British Fertility Society·Justin S LutherJacqueline M Wallace
May 29, 2000·The Proceedings of the Nutrition Society·J M Wallace
Oct 1, 1993·Immunology and Cell Biology·T J O'MearaH W Raadsma
Mar 1, 1990·Australian Veterinary Journal·R J WhittingtonM J Turner

Related Concepts

Antibodies, Bacterial
Antigens, Bacterial
Bacteroides
Cross Reactions
Enzyme-Linked Immunosorbent Assay
Polyglobin
IgM2
Peroxidase-Antiperoxidase Complex Technique
Bacterial Pilus
Dall Sheep

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.