Quantitation of 25-hydroxyvitamin D in dried blood spots by 2D LC-MS/MS without derivatization and correlation with serum in adult and pediatric studies

Clinica Chimica Acta; International Journal of Clinical Chemistry
Berit P JensenChristiaan W Sies

Abstract

Demand for measurement of 25-hydroxyvitamin D (25OHD) is growing and dried blood spot (DBS) sampling is attractive as samples are easier to collect, transport and store. A 2D LC-MS/MS assay without derivatization was developed. DBS punches (3.2 mm) were ultrasonicated with d6-25OHD3 in 70% methanol followed by hexane extraction, dry-down and reconstitution. The assay was validated and applied to two studies comparing whole blood adult DBS with serum samples (n = 40) and neonatal whole blood DBS with cord serum samples (n = 80). The assay was validated in whole blood DBS over the range 13-106 nmol/L 25OHD3 and 11-91 nmol/L 25OHD2 with a limit of detection of 3 nmol/L. Intra- and inter-day imprecision was <13% CV and bias <12%. The assay had high recovery and minimal matrix effects. Triplicate DBS study samples had a mean CV of ≤13% for 25OHD3. No 25OHD2 was detected. DBS calculated serum 25OHD3 concentrations correlated strongly with serum concentrations in the adult DBS/serum study (r = 0.94) and moderately in the neonatal DBS/cord serum study (r = 0.69). Direct quantitation of 25OHD in DBS by 2D LC-MS/MS without derivatization was found to be an alternative to serum quantitation applicable to clinical research studies on adult...Continue Reading

Citations

Jan 8, 2020·European Journal of Clinical Nutrition·Barbara AltieriMichael F Holick
Jul 28, 2019·Clinical Chemistry and Laboratory Medicine : CCLM·Rosita ZakariaRonda F Greaves
Aug 28, 2019·Biomedical Chromatography : BMC·Yan-Yan LiZheng-Chao Ji
Aug 28, 2020·Current Environmental Health Reports·Bryttany McClendon-WearyEdwina Yeung
Mar 22, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hao WangYulan Rao
Mar 14, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Michael J BinksJacobus Ungerer
Apr 2, 2021·Paediatric and Perinatal Epidemiology·Rajneeta SarafCameron C Grant
Apr 21, 2021·Annals of Clinical Biochemistry·Mads NyboKaren Andersen-Ranberg
Sep 3, 2021·Bioanalysis·Vera de KleijneMariëtte T Ackermans

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