PMID: 7026679Jan 1, 1981Paper

Quantitation of a whole blood assay for human natural killer cell activity

Journal of Immunological Methods
P C OttenhofM G Baines

Abstract

A method is described for the measurement of human natural killer cell activity using heparinized whole blood in a 51chromium release assay. Fractionation-reconstitution experiments showed that the cytotoxic activity was abolished by removal of the Fc receptor bearing lymphocytes, but not by the elimination of monocytes and granulocytes. Autologous or pooled plasma was not found to possess inherent cytolytic activity. By analogy to an enzyme kinetic reaction, the results were expressed as kinetic lytic units (KLU) which were defined as the maximum number of target cells that could be lysed per unit time per milliliter of whole blood. The buffy coat cytotoxicity (BCC) assay is quick, easy to perform, and suitable for screening and monitoring of natural cytotoxicity. Since this methodology preserves the actual concentration of natural killer cells, it may represent a truer reflection of in vivo events.

References

Jan 20, 1976·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·C J Sanderson
Nov 15, 1976·International Journal of Cancer. Journal International Du Cancer·H F Pross, M G Baines
Nov 1, 1974·Proceedings of the Society for Experimental Biology and Medicine·O A HoltermannE Klein
Sep 15, 1973·International Journal of Cancer. Journal International Du Cancer·I HellströmG A Warner
Sep 1, 1952·J R Sanit Inst·H ALFORD

❮ Previous
Next ❯

Citations

Feb 1, 1987·The Journal of Clinical Investigation·M PapicM A Fletcher
Dec 18, 2010·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Marián BobovčákJuraj Majtán
Feb 1, 2011·Brain, Behavior, and Immunity·Ben LeviShamgar Ben-Eliyahu
May 1, 1982·The Journal of Urology·A S Pang, A Morales
May 1, 1996·Psychosomatic Medicine·W GerritsenN H Frijda

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.