PMID: 6663488Dec 1, 1983

Quantitation of amikacin, kanamycin, neomycin, and tobramycin in pharmaceutical dosage forms using the Hantzsch reaction

Journal of Pharmaceutical Sciences
V D GuptaJ M Gunter

Abstract

A spectrophotometric assay method for the quantitative determination of amikacin, kanamycin, neomycin, and tobramycin in pharmaceutical dosage forms has been developed. The method is based on the Hantzsch reaction, forming dihydrolutidine derivatives which can be measured spectrophotometrically. The excipients EDTA, phenol, sodium bisulfite, and sodium citrate do not interfere, while salts of ammonia do interfere. The relative standard deviations based on seven readings were 1.64, 1.88, 2.10, and 1.93% for amikacin, kanamycin, neomycin, and tobramycin, respectively. Assay results have been compared with microbiological assay results provided by the manufacturers. The assay method appears to be stability indicating.

References

Feb 1, 1979·The Journal of Pharmacy and Pharmacology·A Csiba
Aug 1, 1979·Antimicrobial Agents and Chemotherapy·E ScarbroughD B Northrop

Citations

Oct 29, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Chia-Hsien FengSu-Hwei Chen
Aug 13, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Mahmoud A OmarSayed M Derayea
Sep 6, 2013·Journal of Chromatographic Science·Mohamed Abdel-Tawab KoranyOsama Ahmed Elmallah
Apr 9, 2021·Journal of Materials Science. Materials in Medicine·Mahima Singh, Sriramakamal Jonnalagadda

Related Concepts

A.M.K
Antibiotics
Microcapsules Drug Delivery System
Drug Formulation Process
Pharmaceutical Excipient
Injectables
Kantrex
Neomycin Palmitate
Solutions
Spectrophotometry

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