PMID: 9532260Apr 9, 1998Paper

Quantitation of bacterial adhesion to polymer surfaces by bioluminescence

Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology
M StollenwerkA Ljungh

Abstract

Quantitation of microbes adhering to a surface is commonly used in studies of microbial adhesion to different surfaces. We have quantified different staphylococcal strains adhering to polymer surfaces by measuring bacterial ATP (adenosine triphosphate) by bioluminescence. The method is sensitive, having a detection limit of 10(4) bacterial cells. Viable counting of bacterial cells may yield falsely low results due to the presence of "dormant" and adherent bacteria. By using bioluminescence, this can be avoided. Cells of different bacterial species and cells of strains of the same species were shown to differ significantly in their basal ATP content (8.7 x 10(-13) - 5.2 x 10(-22) MATP). The size of adherent and planktonic bacteria decreased with time (0.7 micron-->0.3 micron, 20 days). During incubation in nutrient-poor buffer ("starvation"), the ATP content of adherent bacteria decreased after 24-96 h whereas that of planktonic bacteria was stable over 20 days. The presence of human serum or plasma did not interfere significantly with the test results. Since the ATP concentration of bacterial strains of different species varies and is also influenced by the growth conditions of bacteria (solid or liquid culture medium), a speci...Continue Reading

References

Jun 1, 1992·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·M PaulssonT Wadström
Jan 1, 1991·Journal of Medical Microbiology·L Martinez-MartinezE J Perea
Jun 1, 1991·Journal of Medical Microbiology·G Lopez-LopezE J Perea
May 1, 1990·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·F EspersenI Clemmensen
May 1, 1989·Antimicrobial Agents and Chemotherapy·G M Dickinson, A L Bisno
Feb 1, 1993·Journal of Medical Microbiology·M PaulssonA Ljungh

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Citations

Jan 18, 2003·Journal of Microbiological Methods·Britta SwansonJeanine Wiener-Kronish
Jan 5, 2002·Applied and Environmental Microbiology·Hans-Olof NilssonTorkel Wadström
Aug 23, 2011·BMC Microbiology·Miriam HopfeBirgit Henrich
Nov 27, 2001·FEMS Immunology and Medical Microbiology·D Q Li, A Ljungh

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