Quantitation of benzodiazepines in urine, serum, plasma, and meconium by LC-MS-MS

Journal of Analytical Toxicology
Stephanie J MarinGwendolyn A McMillin

Abstract

A single method for confirmation and quantitation of a panel of commonly prescribed benzodiazepines and metabolites, alpha-hydroxyalprazolam, alpha-hydroxyethylflurazepam, alpha-hydroxytriazolam, alprazolam, desalkylflurazepam, diazepam, lorazepam, midazolam, nordiazepam, oxazepam, temazepam, clonazepam, and 7-aminoclonazepam, was developed for three specimen types, urine, serum/plasma, and meconium. Quantitation was by liquid chromatography tandem-mass spectrometry (LC-MS-MS) using a Waters Alliance-Quattro Micro system. The instrument was operated in multiple reaction monitoring mode with an electrospray ionization source in positive ionization mode. The method was evaluated for recovery, imprecision, linearity, analytical measurement range, specificity, and carryover. Average recovery and imprecision (within-run, between-run, and total % CV) were within +/- 15% of the target concentrations for urine (10 to 5000 ng/mL) and serum/plasma (10 to 2500 ng/mL) and within +/- 20% for meconium (10 to 5000 ng/g). In all, 205 patient specimens were analyzed, and the results compared to a previous in-house gas chromatography-MS method or LC-MS-MS results from an outside laboratory. Oxazepam glucuronide was evaluated as a hydrolysis cont...Continue Reading

Citations

Mar 6, 2010·Analytical and Bioanalytical Chemistry·P FernándezP Cabarcos
Mar 27, 2012·Analytical and Bioanalytical Chemistry·Kathrin ArnhardRoland Thiele
Jul 27, 2012·Journal of Analytical Toxicology·Stephanie J MarinGwendolyn A McMillin
Mar 15, 2011·Biomedical Chromatography : BMC·Mitsuhiro Nakamura
Dec 15, 2010·Biomedical Chromatography : BMC·Lewis Couchman, Phillip E Morgan
Aug 16, 2014·Journal of Pharmaceutical and Biomedical Analysis·Koichi SaitoRieko Saito
Feb 26, 2015·Journal of Analytical Toxicology·Giorgio FamigliniAchille Cappiello
Dec 23, 2014·Bioanalysis·Ana Concheiro-Guisan, Marta Concheiro
Sep 15, 2017·Drug Metabolism Reviews·Ricardo Jorge Dinis-Oliveira
May 4, 2016·Antimicrobial Agents and Chemotherapy·Guillermo Martín-GutiérrezJesús Blázquez
Jun 11, 2011·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Juseop KangMin-Young Jun
Jul 1, 2009·Bioanalysis·Victoria F SamanidouIoannis N Papadoyannis
Feb 18, 2020·Journal of Analytical Toxicology·A López-RabuñalA de-Castro-Ríos
May 9, 2009·Analytical Chemistry·T A BrettellJ R Almirall

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