PMID: 9187398Apr 25, 1997Paper

Quantitation of beta-lapachone and 3-hydroxy-beta-lapachone in human plasma samples by reversed-phase high-performance liquid chromatography

Journal of Chromatography. B, Biomedical Sciences and Applications
V L GlenG Wilding

Abstract

beta-Lapachone is an o-naphthoquinone found to have in vitro cytotoxicity in cancer cells, type I human immunodeficiency virus, and fungi. Analytical methods for evaluating beta-lapachone in biological fluids using high-performance liquid chromatography (HPLC) have not been published. The reversed-phase HPLC method described in this report utilizes liquid extraction of a 0.5-ml plasma sample with average recoveries of 67+/-10.8% and 70+/-10.3% for beta-lapachone and 3-hydroxy-beta-lapachone, respectively. Sensitivity of the assay using ultraviolet (UV) detection at 256 nm is 15 ng ml(-1) from a 100 microl injection. Plasma standards for beta-lapachone and 3-hydroxy-beta-lapachone are linear with no significant difference in slope between the compounds. The retention times of 2.7 min for 3-hydroxy-beta-lapachone and 5.9 min for beta-lapachone result in a clean separation permitting use of the same assay procedure without modification for both compounds. This assay offers the advantage that either beta-lapachone or 3-hydroxy-beta-lapachone can serve as the internal standard, depending on which compound is being analyzed.

References

Aug 1, 1984·Journal of Medicinal Chemistry·K Schaffner-SabbaJ W Wasley
Aug 1, 1994·Planta medica·P GuiraudM Simeon de Buochberg
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C J LiA B Pardee

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Citations

May 26, 2012·TheScientificWorldJournal·Marcilio S S Cunha-FilhoMariana Landin
Aug 11, 2011·The Journal of Pharmacy and Pharmacology·Marcílio S S Cunha-FilhoMariana Landín
Aug 20, 2016·European Journal of Drug Metabolism and Pharmacokinetics·Marival BermejoMarta Gonzalez-Alvarez
May 7, 2016·Chemical & Pharmaceutical Bulletin·Ki Hyun KimSeong Hoon Jeong
Feb 24, 2001·Annals of the New York Academy of Sciences·S MiyamotoD A Boothman
Mar 22, 2020·Journal of Bioenergetics and Biomembranes·Caroline Deckmann NicolettiVitor Francisco Ferreira

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