PMID: 2496663May 15, 1989Paper

Quantitation of carbon flow through the hepatic folate-dependent one-carbon pool in rats

Archives of Biochemistry and Biophysics
K L Schalinske, R D Steele

Abstract

The quantitation of carbon flow through the folate-dependent one-carbon pool in regard to the synthesis of methionine from the amino acid precursor, serine, was determined in rat liver. Utilizing duodenal cannulated rats and in vivo tracer kinetic techniques where [3-14C]serine was continuously infused at a rate of 3.32 microCi/h, a steady-state (plateau) specific radioactivity was achieved within 200 min from the onset of the infusion period. This resulted in an irreversible loss rate of 431 +/- 12 mumol/h for hepatic serine. In conjunction with the specific radioactivity measurements of hepatic methionine, the percentage of the total entry into the hepatic methionine methyl carbon pool that came from serine (i.e., transfer quotient) was calculated to be 51.7 +/- 5.2%. Similar experiments utilizing [methyl-3H]methionine as the infusate resulted in a value of 112 +/- 6 mumol/h for the irreversible loss rate of hepatic methionine. Using the irreversible loss rate of methionine and the transfer quotient to methionine from serine, the flux of the beta-carbon of serine to remethylate homocysteine and generate methionine was calculated to be 57.9 mumol/h. These results not only present a methodology for the determination of folate-d...Continue Reading

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Citations

Oct 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·D K HansenT F Grafton
Apr 4, 2012·Environmental Health Perspectives·Mary V Gamble, Megan N Hall
May 25, 2013·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·John C PanettaWilliam E Evans
Jun 21, 2006·Metabolism: Clinical and Experimental·Yoshihiko ShinoharaTakao Hashimoto
Nov 15, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Justin H TownsendJesse F Gregory
Oct 16, 2003·American Journal of Physiology. Endocrinology and Metabolism·Steven R DavisJesse F Gregory
Mar 10, 2005·The American Journal of Clinical Nutrition·Steven R DavisJesse F Gregory
Nov 10, 2001·American Journal of Physiology. Endocrinology and Metabolism·G J CuskellyJ F Gregory
Apr 28, 2021·Toxicology·Ahlam AbuawadMary V Gamble

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