Quantitation of covalently closed circular hepatitis B virus DNA in chronic hepatitis B patients

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Danny Ka-Ho WongChing-Lung Lai

Abstract

This study examined a signal amplification assay, the Invader assay, for the quantitation of hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) in liver biopsies and sera. DNA was extracted from liver biopsy and serum samples were collected from 16 hepatitis B e antigen (HBeAg)-positive and 36 antibody-to-HBeAg-positive (anti-HBe-positive) chronic hepatitis B patients. The amount of total HBV DNA and cccDNA was measured using the Invader assay. Anti-HBe-positive patients had lower median total intrahepatic HBV DNA (P < .001) and intrahepatic cccDNA levels (P = .001) than HBeAg-positive patients. Intrahepatic cccDNA correlated positively with the total intrahepatic HBV DNA (r = 0.950, P < .001). However, the proportion of intrahepatic HBV DNA in the form of cccDNA was inversely related to the amount of total intrahepatic HBV DNA (r = -0.822, P < .001). A small amount of cccDNA was detected in 39 of 52 (75%) serum samples. Anti-HBe-positive patients had lower median serum cccDNA levels than HBeAg-positive patients (P = .002). Serum HBV DNA correlated positively with intrahepatic total HBV DNA (r = 0.778, P < .001) and intrahepatic cccDNA (r = 0.481, P = .002). In conclusion, the Invader assay is a reliable assay for ...Continue Reading

References

Nov 1, 1992·Journal of Gastroenterology and Hepatology·W S Robinson
Sep 1, 1981·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R G KnodellJ Wollman
Mar 1, 1996·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J KöckH J Schlicht
Jan 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C L LaiP C Wu
Dec 11, 1997·The New England Journal of Medicine·W M Lee
Jun 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A L MasonR P Perrillo
Sep 25, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J L DienstagN Brown
Feb 15, 2000·Molecular Diagnosis : a Journal Devoted to the Understanding of Human Disease Through the Clinical Application of Molecular Biology·R W KwiatkowskiB Neri
Mar 8, 2000·Microbiology and Molecular Biology Reviews : MMBR·C Seeger, W S Mason
Jun 28, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M CabrerizoV Carreno
Jul 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·J G HallV I Lyamichev
Nov 16, 2001·Annals of the New York Academy of Sciences·R BreitkreutzC H Schröder
Dec 4, 2001·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A S LokUNKNOWN Practice Guidelines Committee, American Association for the Study of Liver Diseases
May 23, 2002·Gastroenterology·Jean-Michel Pawlotsky
Jul 10, 2003·Gastroenterology·Christian KleinChristian Trautwein
Jul 15, 2003·Journal of Medical Virology·Wei ZhangClaus H Schröder
Oct 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yong-Yuan ZhangJesse Summers
Jan 8, 2004·Journal of Hepatology·Jean-Michel Pawlotsky
Mar 5, 2004·Journal of Viral Hepatitis·M-C LiuG-Q Wang
Jun 9, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Man-Fung YuenChing-Lung Lai

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Citations

May 1, 2008·Hepatology International·D Scott Bowden, Alex J Thompson
Jul 26, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kristie BloomPatrick Arbuthnot
Oct 4, 2011·The Journal of Antimicrobial Chemotherapy·James FungMan-Fung Yuen
Sep 9, 2005·Current Opinion in Infectious Diseases·Alex Yui Hui, Joseph Jao-Yiu Sung
May 11, 2007·Journal of Gastroenterology and Hepatology·Man-Fung Yuen
Jul 17, 2007·Clinical Microbiology Reviews·Alexandra Valsamakis
Oct 19, 2007·Journal of Clinical Microbiology·Danny Ka-Ho WongMan-Fung Yuen
Mar 20, 2015·Jundishapur Journal of Microbiology·Payam BehzadiSeyed Moayed Alavian
Jun 4, 2014·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Andrzej TarantaKarsten Wursthorn
Feb 13, 2010·European Journal of Gastroenterology & Hepatology·R Bart TakkenbergMarcel G H M Beld
Dec 8, 2015·Experimental and Therapeutic Medicine·Hongyu JiaLinfu Zhou
Sep 29, 2015·Journal of Gastroenterology and Hepatology·C Nelson HayesKazuaki Chayama
Aug 3, 2010·Infectious Disease Clinics of North America·Lynne Strasfeld, Sunwen Chou
May 15, 2010·Infectious Disease Clinics of North America·Lynne Strasfeld, Sunwen Chou
Oct 11, 2008·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Emmet B KeeffeHillel Tobias
Apr 28, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Tsuyoshi HatakeyamaKazuaki Chayama
Dec 1, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Maura Dandri, Jorg Petersen
Aug 31, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Andreas LarasStephanos J Hadziyannis
Jul 19, 2015·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Paul MartinIra M Jacobson
Aug 26, 2014·Biochemical and Biophysical Research Communications·Naoki OguraTakaji Wakita
Jul 21, 2009·Journal of Hepatology·Massimo LevreroMaura Dandri
Dec 20, 2016·Liver International : Official Journal of the International Association for the Study of the Liver·Danny Ka-Ho WongMan-Fung Yuen
Jun 12, 2014·United European Gastroenterology Journal·Cosmas Rinaldi Adithya LesmanaScott David Bowden

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