Quantitation of Cremophor EL in human plasma samples using a colorimetric dye-binding microassay

Analytical Biochemistry
A SparreboomK Nooter

Abstract

This paper describes an analytical procedure for the quantitative determination of the pharmaceutical vehicle Cremophor EL in human plasma samples. The procedure is based on rapid binding of Coomassie brilliant blue G-250 to Cremophor EL following plasma protein precipitation with acetonitrile and analyte extraction with n-butylchloride. The binding of the dye to Cremophor EL causes a shift in the absorption maximum from 465 to 624 nm, which is monitored using an automated microplate-absorbance reader. The assay permits estimation of Cremophor EL concentrations in the range 0.05-1.00% (v/v) in 50 microL of human plasma, with percentage deviation and precision of < or = 12 and < or = 15%, respectively. The assay was subsequently used to measure Cremophor EL concentrations in plasma samples in support of a project to develop a pharmacokinetic model for this compound in patients receiving paclitaxel.

References

Mar 1, 1994·Journal of Photochemistry and Photobiology. B, Biology·K Woodburn, D Kessel
Jan 1, 1996·Anti-cancer Drugs·A SparreboomJ H Beijnen

❮ Previous
Next ❯

Citations

Jun 4, 2008·Biological & Pharmaceutical Bulletin·Xiang-Rui LiuQiang Zhang
Aug 29, 2013·International Journal of Analytical Chemistry·V Vijaya Bhaskar, Anil Middha
Feb 14, 2006·Seminars in Oncology·Frederick H HausheerFrank Lieberman
Jan 15, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Carolien H SmorenburgJaap Verweij
Jan 12, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Hans GelderblomAlex Sparreboom
Jan 18, 2003·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Z TabarelliM A Rubin
Feb 17, 2017·Chemical Biology & Drug Design·Alejandra Martinez De Pinillos BayonaMichael R Hamblin
Jul 29, 2016·Journal of Oncology Pharmacy Practice : Official Publication of the International Society of Oncology Pharmacy Practitioners·Jean-Philippe AdamNathalie Letarte
Dec 17, 2008·Photochemistry and Photobiology·Sunita Patel, Anindya Datta
Sep 25, 1999·British Journal of Cancer·O van TellingenJ H Beijnen
Jul 8, 2003·Clinical Pharmacokinetics·Albert J ten TijeAlex Sparreboom
Jul 13, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Isabel Durán Martín-MerásMaría del Carmen Hurtado-Sánchez
Sep 21, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·William J GradisharJoyce O'Shaughnessy
Sep 2, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Nuhad K IbrahimGabriel N Hortobagyi
Oct 16, 2001·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·A HenningssonA Sparreboom

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.