PMID: 9438448Jan 23, 1998Paper

Quantitation of dexfenfluramine/d-norfenfluramine concentration in primate brain using 19F NMR spectroscopy

Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine
J D ChristensenP F Renshaw

Abstract

Fluorine (19F) magnetic resonance spectroscopy (MRS) was used to quantify the combined concentration of the anorectic drug dexfenfluramine (DF) and its active metabolite d-norfenfluramine (dNF) in rhesus monkey brain. The accuracy of the MRS technique was assessed by comparison with gas chromatography. Brain 19F MRS signals were converted to brain DF + dNF concentrations after correction for signal relaxation losses and drug distribution in nonbrain tissue. Gas chromatography (GC) was used to assay brain DF and dNF concentrations following MRS evaluation. DF + dNF concentrations measured by 19F MRS averaged 104 +/- 36 microM (mean +/- SD) and GC measurements averaged 71 +/- 12 microM. Correction for the distribution of DF and its metabolites in nonbrain tissue yielded a DF + metabolite brain concentration that was within one standard deviation of the GC-derived value. The concentration of DF plus dNF measured by 19F MRS was similar to or greater than the value obtained by GC, which indicates that DF and its active metabolite dNF are fully detected by 19F MRS in primate brain in vivo. The application of these techniques to human subjects should enable the measurement of low micromolar-range brain concentrations of DF and other f...Continue Reading

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Citations

Feb 15, 2005·Magnetic Resonance Imaging·Josef PfeufferNikos K Logothetis
Mar 13, 2002·Pharmacology, Biochemistry, and Behavior·Richard B Rothman, Michael H Baumann
Aug 7, 2002·Pharmacology & Therapeutics·Richard B Rothman, Michael H Baumann
May 12, 2005·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Michael E HenryPerry F Renshaw
Feb 13, 2002·Biological Psychiatry·In Kyoon Lyoo, Perry F Renshaw

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