Quantitation of DNA and RNA with absorption and fluorescence spectroscopy

Current Protocols in Neuroscience
S R Gallagher

Abstract

Reliable quantitation of nanogram and microgram amounts of DNA and RNA in solution is essential to researchers in molecular biology. In addition to the traditional absorbance measurements at 260 nm, two more sensitive fluorescence techniques are presented below. These three procedures cover a range from 5 to 10 ng/ml DNA to 50 mg/ml DNA.

Citations

Dec 15, 2020·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Jinzhi LvYanming Miao

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