Quantitation of fetal-maternal hemorrhage by flow cytometry. A simple and accurate method

American Journal of Clinical Pathology
S J NanceG Garratty

Abstract

A simple and objective assay was developed for the detection and quantitation of fetal-maternal hemorrhage with the use of flow cytometry. In vitro prepared control mixtures of 10%, 2%, 1%, 0.5%, 0.25%, 0.125%, and 0.06% D+ RBCs in D- RBCs were tested (8-11) different times by flow cytometry and gave mean % D+ results of 11.10%, 1.90%, 0.92%, 0.45%, 0.24%, 0.11%, and 0.05%. The coefficient of variation of preparing and testing these mixtures ranged from 11.0 to 15.9% for the 10-0.125% mixtures. Thus, flow cytometry was accurate, reproducible, and sensitive. Flow cytometry was compared with Du tests, rosette tests, and acid elution. The Du test was highly variable because it was not sensitive enough to detect a significant bleed (approximately 0.6%) in some cases and too sensitive (necessitating quantitation of an insignificant bleed) in others. The rosette test was too sensitive. Acid elution and flow cytometry results did not always agree; acid elution results were approximately twice as high as flow cytometry. The authors believe flow cytometric detection of D+ red blood cells to be more accurate than the detection of fetal hemoglobin by acid elution techniques, which is known to have poor reproducibility. Postpartum samples ...Continue Reading

Citations

Dec 10, 1999·Cytometry·G Garratty, P A Arndt
Feb 14, 2004·Transfusion·John D RobackChristopher D Hillyer
May 30, 2008·American Journal of Hematology·Patricia A Arndt, Belinda M Kumpel
Mar 5, 2011·Transfusion Medicine·B NatukundaH Schonewille
Sep 16, 2011·American Journal of Clinical Pathology·Harry de WitJohannes J M L Hoffmann
Nov 22, 2012·Obstetrics and Gynecology·S Gerald Sandler, Jerome L Gottschall
Jun 26, 2003·Transfusion·John D RobackChristopher D Hillyer
May 3, 2002·Clinical and Laboratory Haematology·W C M Janssen, J J M L Hoffmann
Sep 5, 2008·Current Protocols in Cytometry·Bruce H Davis, Kathleen Thompson Davis
Oct 21, 2006·Current Opinion in Hematology·Morten Hanefeld DziegielAdela Berkowicz
Jun 27, 2003·Cytometry. Part B, Clinical Cytometry·Denise M V PelikanHans J Tanke
Jan 30, 1999·Transfusion Medicine Reviews·J K Duguid, I M Bromilow
Apr 1, 1995·Transfusion Medicine Reviews·J Freedman, A H Lazarus

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