Quantitation of flavanols, proanthocyanidins, isoflavones, flavanones, dihydrochalcones, stilbenes, benzoic acid derivatives using ultraviolet absorbance after identification by liquid chromatography-mass spectrometry.

Journal of Agricultural and Food Chemistry
Long-Ze Lin, James M Harnly

Abstract

A general method was developed for the systematic quantitation of flavanols, proanthocyanidins, isoflavones, flavanones, dihydrochalcones, stilbenes, and hydroxybenzoic acid derivatives (mainly hydrolyzable tannins) based on UV band II absorbance arising from the benzoyl structure. The compound structures and the wavelength maximum were well correlated and were divided into four groups: the flavanols and proanthocyanidins at 278 nm, hydrolyzable tannins at 274 nm, flavanones at 288 nm, and isoflavones at 260 nm. Within each group, molar relative response factors (MRRFs) were computed for each compound based on the absorbance ratio of the compound and the group reference standard. Response factors were computed for the compounds as purchased (MRRF), after drying (MRRFD), and as the best predicted value (MRRFP). Concentrations for each compound were computed based on calibration with the group reference standard and the MRRFP. The quantitation of catechins, proanthocyanidins, and gallic acid derivatives in white tea was used as an example.

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Citations

Jan 1, 2016·Journal of Chromatography. a·André de VilliersHarald Pasch
Mar 31, 2015·Journal of Food Science·Leila SaldanhaSushma Savarala
Jun 19, 2015·Nutrition Reviews·Julia J PetersonMarjorie L McCullough
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Mar 5, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wan-Yi GuJian-Lin Wu
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Apr 10, 2015·The American Journal of Clinical Nutrition·Douglas A BalentineCatherine L Kwik-Uribe
Sep 3, 2020·Analytical and Bioanalytical Chemistry·Yifei WangPei Chen
Nov 30, 2018·Environmental Science and Pollution Research International·Sihem GuesmiHaitham Sghaier
Jul 19, 2018·Current Developments in Nutrition·Gloria I Solano-AguilarCindy D Davis
Aug 31, 2020·Journal of Food Composition and Analysis : an Official Publication of the United Nations University, International Network of Food Data Systems·Yifei WangPei Chen
Sep 20, 2017·Journal of Food Composition and Analysis : an Official Publication of the United Nations University, International Network of Food Data Systems·Mengliang ZhangPei Chen
Jul 18, 2014·Journal of Agricultural and Food Chemistry·Long-Ze LinJames M Harnly

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