Quantitation of fungal DNA contamination in commercial zymolyase and lyticase used in the preparation of fungi

Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology
Yoshiharu MiyajimaKoichi Makimura

Abstract

Small amounts of contaminants may lead to false-positive results in sensitive polymerase chain reaction (PCR) detection systems. To analyze contaminants and understand the usability of beta-glucanases in fungal preparations, we estimated the ribosomal DNA (rDNA) contamination in Zymolyase-100T and Lyticase by quantitative PCR. The amount of rDNA contamination determined by real-time PCR was 9210 copies/unit for Zymolyase-100T and 0.0323 copies/unit for Lyticase. The observations regarding these enzyme products indicate that careful consideration of contaminating DNA included in the reagents used for molecular diagnostics is necessary.

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Citations

Dec 17, 2015·Antonie van Leeuwenhoek·Liangzhi LiBingyu Tang
Jul 2, 2014·Clinical Microbiology Reviews·Marios ArvanitisEleftherios Mylonakis
Mar 13, 2021·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Shawn R LockhartDimitrios P Kontoyiannis
Oct 12, 2016·Microbiology Spectrum·Sean X Zhang, Nathan P Wiederhold

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