PMID: 6166860Jun 1, 1981Paper

Quantitation of genetic differences between Trypanosoma brucei gambiense, rhodesiense and brucei by restriction enzyme analysis of kinetoplast DNA

Molecular and Biochemical Parasitology
P BorstW C Gibson

Abstract

We have compared a total of 44 recognition sites for 12 restriction endonucleases on the 20 kilobase pair maxi-circle of kinetoplast DNA from nine Trypanosoma brucei stocks, four which are known to be infective to man (tow 'gambiense' and two 'rhodesiense' variants). In addition to five polymorphic sites, these DNAs differ in the size of a 5 kilo-base pair region which is cleaved only by one of the restriction enzymes tested and which varies in size over 1.5 kilo-base pairs. Our analysis shows that the maxi-circle sequences of these stocks are very similar, the maximal calculated difference between any two being 3%. A relatively large difference was found between a rhodesiense stock from uganda and one from Zambia, confirming the distinction between northern and southern East African rhodesiense stocks found by analysis of enzyme polymorphisms (Gibson et al. (1980) Adv. Parasitol. 18, 175-246). The gambiense variants could not be identified by unique restriction site polymorphisms, but contained the smallest maxi-circle found thus far in T. Brucei. Our results indicate that T. brucei stocks infective and not infective to man are so closely related as to preclude their differentiation by analysis of kinetoplast DNA. This analysi...Continue Reading

References

Jun 5, 1978·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·G A Cross
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·W M BrownA C Wilson
Jun 1, 1976·The Journal of General Virology·L V Crawford, A K Robbins
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M Nei, W H Li
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J C AviseR A Lansman
Jan 1, 1979·Plasmid·P Borst, J H Hoeijmakers
Nov 22, 1979·Biochimica Et Biophysica Acta·P Borst, F Fase-Fowler
Sep 15, 1977·Journal of Molecular Biology·B S Zain, R J Roberts
Apr 1, 1978·Nucleic Acids Research·J S SussenbachJ D van Embden
Jun 22, 1978·Nature·K Vickerman
Feb 24, 1977·Nature·F SangerM Smith
May 5, 1976·Journal of Molecular Biology·R J RobertsK Murray
Feb 25, 1975·Journal of Molecular Biology·R OldG Boizes
Jan 25, 1975·Journal of Molecular Biology·M Thomas, R W Davis
Jun 4, 1974·Biochemical and Biophysical Research Communications·F M DeFilippes
Dec 1, 1980·Molecular and Biochemical Parasitology·A M Simpson, L Simpson
May 1, 1981·The Journal of protozoology·R S Cuthbertson

❮ Previous
Next ❯

Citations

Feb 15, 1991·Experientia·G Hide, A Tait
May 1, 1993·Acta Tropica·P J Myler
Oct 1, 1983·Molecular and Biochemical Parasitology·K Stuart
Jul 1, 1984·Molecular and Biochemical Parasitology·J M NtambiP T Englund
Sep 1, 1985·Molecular and Biochemical Parasitology·W C GibsonP Borst
May 1, 1985·Molecular and Biochemical Parasitology·P BorstK Vickerman
Feb 1, 1987·Molecular and Biochemical Parasitology·P BorstW C Gibson
Sep 1, 1989·Molecular and Biochemical Parasitology·C De GreefR Hamers
Aug 1, 1990·Molecular and Biochemical Parasitology·W Gibson, L Garside
Dec 1, 1992·Molecular and Biochemical Parasitology·S T LeeC Y Wang
Dec 11, 2002·Trends in Parasitology·Wendy Gibson
Feb 1, 1984·Parasitology·N N Massamba, R O Williams
Jun 27, 2009·Parasitology·G Hide, A Tait
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M L MuhichA M Simpson
Nov 15, 2000·Proceedings of the National Academy of Sciences of the United States of America·A MacLeodA Tait
Sep 9, 2016·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·K P ShymaVeer Singh
Nov 1, 1986·The Journal of protozoology·J L AffranchinoA O Stoppani
Feb 1, 1991·Annals of Tropical Medicine and Parasitology·P DukesD G Godfrey
Feb 1, 1992·Annals of Tropical Medicine and Parasitology·J R StevensJ K Gashumba
Jun 1, 1982·Cell·P Borst, G A Cross
Jan 1, 1984·Journal of Cellular Biochemistry·T W SpithillG F Mitchell
Jun 15, 1983·Journal of Molecular Biology·P SloofW Leon

❮ Previous
Next ❯

Related Concepts

Related Feeds

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.