PMID: 2100208Jan 1, 1990Paper

Quantitation of human chorionic gonadotrophin (hCG) by radioreceptor assay

Journal of Pharmaceutical and Biomedical Analysis
L SjödinE Viitanen

Abstract

A sensitive radioreceptor assay was developed for pharmaceutical preparations of human chorionic gonadotrophin with the use of rat testicular membranes as receptor preparation and human 125I-chorionic gonadotrophin as tracer. The addition of unlabelled human chorionic gonadotrophin or luteinizing hormone inhibited the binding of 125I-chorionic gonadotrophin to the receptors in a concentration dependent way. Concentrations of human chorionic gonadotrophin between 30-300 mIU ml(-1) were normally used for a three-dose assay fulfilling pharmacopoeial statistical requirements for assay validity. The relative standard deviation for five assays was 7%. Estimates of potency of commercial preparations of human chorionic gonadotrophin obtained with the radioreceptor assay correlated well with corresponding estimates from in vivo assays. The proposed radioreceptor assay, however, provides a considerable saving in the number of animals required, requires less technical support, and is more precise than the in vivo method.

References

Jun 1, 1989·The Journal of Pharmacy and Pharmacology·L SjödinE Viitanen
Jul 1, 1985·Journal of Biological Standardization·L Sjödin
Jun 1, 1987·Pharmaceutical Research·L Sjödin, E Viitanen
Jul 1, 1987·Journal of Biological Standardization·T Nederman, L Sjödin
Dec 21, 1971·Biochimica Et Biophysica Acta·M L DufauT Tsuruhara
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Sep 1, 1980·Analytical Biochemistry·P J Munson, D Rodbard

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Citations

Sep 13, 2005·Journal of Immunoassay & Immunochemistry·Pramod V PrasadK Muralidhar

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