PMID: 6975790Jan 1, 1981Paper

Quantitation of human complement fragment C4ai in physiological fluids by competitive inhibition radioimmune assay

Journal of Immunological Methods
J P Gorski

Abstract

A method is described to quantitate complement fragment C4ai in human plasma, synovial fluid, and urine. Samples are first precipitated with 50% saturated (NH4)2SO4 to remove cross-reactive macromolecules C4 and pro-C4. Whereas greater than 97% of C4 is removed by this precipitation step, 88% of C4ai remains in solution. Second, the concentration of C4ai in supernatant fractions is determined by double antibody competitive inhibition radioimmunoassay. C4a was recently completely sequenced (Moon et al., 1981) and is readily available as a pure standard. Examination of the specificity of this method confirmed it was indeed specific for C4a antigenicity. Immunochemically depleted C4-deficient plasma and inulin-activated reconstituted C4-deficient plasma exhibited less than 0.1% of the immunoreactivity of untreated plasma. In addition, good agreement was observed in analyses of aggregated IgG activated serum between the experimentally determined concentration of C4ai and that expected from the initial concentration of C4. As a result, recovery and measurement of C4ai in physiological fluids with this method appear both quantitative and specific. Based on results from 17 adult volunteers, the average concentration of C4ai in normal ...Continue Reading

References

Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·D E Chenoweth, T E Hugli
Dec 1, 1979·Annals of Internal Medicine·J G CurdJ H Vaughan
Nov 1, 1974·The Journal of Experimental Medicine·R D Schreiber, H J Müller-Eberhard
Sep 28, 1972·The New England Journal of Medicine·S RuddyK F Austen
Jan 1, 1974·Nephron·W Strober, T A Waldmann
Jan 1, 1968·Annual Review of Medicine·P H Schur, K F Austen
Jun 1, 1967·The Journal of Clinical Investigation·M J Chamberlain, L Stimmler
Nov 1, 1980·The Journal of Allergy and Clinical Immunology·H G Morris
Feb 26, 1981·The New England Journal of Medicine·D E ChenowethJ W Kirklin

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Citations

Feb 1, 1991·Journal of Biomedical Materials Research·P G KalmanA D Romaschin
Jan 1, 1996·Surgery Today·H NakaeM Yoshida
Jul 1, 1988·Molecular Immunology·L CuiT E Hugli
Sep 1, 1992·Immunopharmacology·M OppermannO Götze
Sep 1, 1987·Journal of the American College of Cardiology·W R BennettR Bolli
Oct 4, 1984·The New England Journal of Medicine·R M HakimF K Port
Apr 1, 1989·Catheterization and Cardiovascular Diagnosis·R L ClickA A Bove
Sep 1, 1995·Clinical and Diagnostic Laboratory Immunology·A E Ahmed, J B Peter

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