Quantitation of human cytomegalovirus DNA in plasma using an affordable in-house qPCR assay

Journal of Virological Methods
Behzad KhansarinejadYadollah Sanahmadi

Abstract

Quantitation of human cytomegalovirus (HCMV) DNA is used to monitor immunocompromised patients in order to identify patients for preemptive therapy. Although several commercial qPCR assays are available for quantitation of HCMV, their major disadvantage is the high cost. In the present study, an internally controlled quantitative real-time PCR assay based on hydrolysis probe technology was developed for detection and quantitation of HCMV DNA in plasma samples. To demonstrate its performance characteristics, a total of 178 plasma samples from 102 kidney and hematopoietic stem cell transplanted patients were tested. The assay showed good precision and reproducibility, and an analytical sensitivity of 288.5 copies/ml or 17.6 copies/reaction. A sensitivity of 93.1% and a specificity of 96.6% were determined by examining clinical samples. Analysis of a panel containing potentially interfering viruses demonstrated no cross-reactivity with the assay. A strong correlation was observed between this qPCR method and the commercial Artus(®) CMV RG PCR kit (R=0.948; P=0.000). These results indicate that the affordable internally controlled qPCR method described will be useful for monitoring HCMV infection in plasma samples of immunocompromi...Continue Reading

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Citations

Nov 23, 2013·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Nyaxewo MwaanzaMatthew Bates
Mar 14, 2013·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Yao-Ming ChenWen-Chien Ko
Jun 17, 2014·Microbiology and Immunology·Haiyan XuXueguang Zhang
Feb 24, 2015·Transplant International : Official Journal of the European Society for Organ Transplantation·Behzad KhansarinejadMahdieh Mondanizadeh
Oct 30, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·John TemboMatthew Bates
Aug 10, 2013·Pathogens and Disease·Wioletta WujcickaDorota Nowakowska
Feb 4, 2021·Infectious Agents and Cancer·Mehrdad PayandehMohammad Dehghannejad

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