PMID: 6966315May 1, 1980Paper

Quantitation of influenza virus antigens on infected target cells and their recognition by cross-reactive cytotoxic T cells

The Journal of Experimental Medicine
C J HackettK van Wyke

Abstract

Monoclonal antibody to type-A influenza virus matrix (M)-protein was used to quantitate the appearance of M-protein on abortively infected P815 cells. After 16 h of infection with different type-A viruses, only a low amount of M-protein appears on the surface of infected cells (approximately 10(3) site/cell) in contrast to approximately 10(5) hemagglutinin molecules on each cell surface. However, virus replication is required for M-protein appearance. Analysis of solubilized membranes purified from 16-h-infected cells shows approximately 10(4) M-protein molecule/cell in the plasma membrane, a content that is consistent with the observed low surface expression, and that indicates that most of the M-protein is localized internally. We found no evidence that cross-reactive cytotoxic T cells could recognize M-protein; neither monoclonal antibody or hyperimmune anti-M-protein antiserum could inhibit T cell killing, either alone or in combination with monoclonal anti-H-2 antibody. Taken together, the low level of M-protein appearance and lack of T cell blocking by anti-M-protein antibody leaves doubt that M-protein is the antigen recognized by cross-reactive cytotoxic T cells.

References

Jul 1, 1976·European Journal of Immunology·E SimpsonP Chandler
Oct 1, 1978·European Journal of Immunology·A J McMichael, B A Askonas
Mar 29, 1979·Nature·J C Howard, J R Corvalan

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Citations

Oct 1, 1984·European Journal of Clinical Microbiology·R A Polin
Dec 1, 1981·In Vitro·R H Kennett
Sep 1, 1985·Cellular Immunology·G SterkersJ P Lévy
Mar 1, 1983·Veterinary Immunology and Immunopathology·D E Onions
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J W YewdellB Moss
Jul 1, 1982·The Journal of Experimental Medicine·M W ShawR W Compans
Jan 1, 1981·Immunological Reviews·R M Zinkernagel, K L Rosenthal
Dec 1, 1981·Infection and Immunity·M W ShawR W Compans

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