Quantitation of lysozyme peptides bound to class II MHC molecules indicates very large differences in levels of presentation

The Journal of Immunology : Official Journal of the American Association of Immunologists
C VelazquezE R Unanue

Abstract

Knowing the abundance of peptides presented by MHC molecules is a crucial aspect for understanding T cell activation and tolerance. In this report we determined the relative abundance of four distinct peptide families after the processing of the model Ag hen egg-white lysozyme. The development of a sensitive immunochemical approach reported here made it possible to directly quantitate the abundance of these four epitopes presented by APCs, both in vitro and in vivo. We observed a wide range of presentation among these four different epitopes presented on the surface of APCs, with 250-fold differences or more between the most abundant epitope (48-63) and the least abundant epitopes. Importantly, we observe similar ratios of presentation from APCs in vitro as well as from APCs from the spleens and thymi of hen egg-white lysozyme transgenic mice. We discuss the relationship between the amount of peptide presented and their binding to I-A(k) molecules, immunogenicity, and tolerogenicity.

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Citations

Jun 11, 2002·The Journal of Biological Chemistry·Carlos VelazquezEmil R Unanue
Sep 10, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Richard J DiPaolo, Emil R Unanue
May 22, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ravi K VeeraswamyEmil R Unanue
Sep 27, 2005·Immunological Reviews·Scott B Lovitch, Emil R Unanue
Aug 4, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chiao-Wen YangEmil R Unanue
Jun 27, 2002·Proceedings of the National Academy of Sciences of the United States of America·Zheng PuEmil R Unanue
Oct 23, 2002·The Journal of Experimental Medicine·Evelyne MougneauNicolas Glaichenhaus
Feb 8, 2003·Environmental Health Perspectives·Marcia SticklerFiona Harding
May 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jeremy HerzogEmil R Unanue
Jun 11, 2011·Immunogenetics·Robert L SchelonkaHarry W Schroeder
Dec 19, 2012·Proceedings of the National Academy of Sciences of the United States of America·Vijay VanguriEric S Huseby
May 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·V Peter YeungFiona A Harding
May 9, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·D A PetersonE R Unanue
Jan 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anish SuriEmil R Unanue
Aug 23, 2002·Immunological Reviews·Emil R Unanue
Jun 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Richard J DiPaolo, Emil R Unanue
Aug 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Scott B LovitchEmil R Unanue
Nov 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Craig A ByersdorferEmil R Unanue
May 20, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·F Harding
Nov 26, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Danielle F AtibalentjaEmil R Unanue
Apr 29, 2014·Molecular Cell·Michael L Dustin

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