PMID: 6164246Dec 1, 1980Paper

Quantitation of M antigen in Lancefield extracts of group A streptococci type 12 using electro-immuno assay

Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology
I EliassonP Christensen

Abstract

Anti-type 12 serum incorporated in agarose-polyethylene glycol gel in a concentration of 1.5% (vol/vol) was found to enable a distinct "rocket" precipitate in electro-immuno assay using hot hydrochloric acid extract of type 12 group A streptococci. This precipitate was removed by trypsin treatment of the extract and on addition of anti-M12 typing serum but not of five other typing sera to the extract. The streptococcal component responsible for this precipitate was eluted from a CM-cellulose ion exchange column at pH 6.5. These findings demonstrated that the precipitate was caused by the M12 antigen. Crossed immuno-electrophoresis of hot hydrochloric acid extracts of three different type 12 group A streptococci showed that the electrophoretic mobility of the M12 antigens was similar in the three extracts. A linear correlation was obtained between the concentration of the M12-antigen and the height of the precipitate obtained in the electro-immuno assay using different dilutions of a standard type 12 extract. M12 antigen could thus be quantitated by the electro-immuno assay. In quantitation experiments, uniformly prepared extracts of five randomly selected, freshly-isolated type 12 strains were found to contain from 130 to 1850%...Continue Reading

References

Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·E H BeacheyA H Kang
Jan 1, 1972·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·C B Laurell
Oct 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·E N Fox, M K Wittner
Feb 1, 1965·Analytical Biochemistry·C B LAURELL
Nov 30, 1928·The Journal of Experimental Medicine·E W Todd, R C Lancefield

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