PMID: 3510670Jan 15, 1986

Quantitation of muscle glycogen phosphorylase mRNA and enzyme amounts in adult rat tissues

Biochimica Et Biophysica Acta
E S David, M M Crerar

Abstract

Mammalian glycogen phosphorylases comprise a family of isozymes that are expressed selectively in a variety of cell types. As an initial step towards understanding the molecular processes that regulate the differential expression of the phosphorylase family, we have begun a quantitative examination of isozyme expression in vivo. In this paper, we report quantitative estimates of the amounts of the muscle (M) isozyme and its mRNA in adult rat tissues. Quantitative estimates of the amount of M-phosphorylase were obtained by an analysis involving electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose filters and sequential treatment with M-isozyme specific antibody and radioactively- labeled protein A. M-phosphorylase mRNA amounts were determined by an analysis involving transfer of RNA from agarose gels to nitrocellulose filters and subsequent hybridization with radioactively labelled rat M-phosphorylase cDNA. These studies indicate that M-phosphorylase is present in all tissues tested with the possible exception of liver. These are skeletal muscle, heart, brain, stomach, lung, kidney, spleen and testis. Quantitation of M-phosphorylase amounts indicate that there is a wide spectrum of variation (over 1000...Continue Reading

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Jan 1, 1988·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·V DombrádiL N Johnson
Jun 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S Scapin, G Di Giuseppe
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C B NewgardR Fletterick
Aug 30, 2008·Neurochemical Research·Heide SchmidBernd Hamprecht
Jan 29, 2008·Journal of Neurochemistry·Anne B WallsHelle S Waagepetersen
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Jul 24, 2014·Journal of Inherited Metabolic Disease·Gisela Nogales-GadeaTomàs Pinós
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Jul 25, 2018·Cells·Marcelina ParraHanjo Hellmann
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Mar 21, 2019·Journal of Neuroscience Research·Long WuR A Swanson
Feb 16, 2008·Neurochemical Research·Gustavo Sánchez-ChávezRocío Salceda

Related Concepts

Metazoa
Antibody Specificity
DNA, Double-Stranded
SDS-PAGE
Phosphorylases
Antibody Dissociation
Alloenzymes
Muscle
Genomic Hybridization
Poly(A) Tail

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