PMID: 7514800Feb 24, 1994Paper

Quantitation of N-terminally extended tachykinins in cerebrospinal fluid from healthy subjects

Regulatory Peptides
G ToressonL Bertilsson

Abstract

N-terminally extended substance P (SP) and neuropeptide K (NPK), an N-terminally extended form of neurokinin A (NKA), were determined in cerebrospinal fluid (CSF) from healthy human subjects by combined high performance liquid chromatography and radioimmunoassay. The concentrations of the peptides were similar in fresh CSF and in CSF which had been kept frozen for up to 5 months. SP and NKA were not present in measurable amounts in neither fresh CSF nor in CSF that had been frozen. On the other hand, when synthetic SP and NKA were added to approx. 2 pM concentration to fresh CSF samples, both peptides were recovered to 85 and 98%, respectively. There were no significant concentration gradients of the peptides in the first 18 ml (three consecutive 6 ml fractions) of CSF (n = 10). In contrast, we confirmed previous findings, that there are gradients of the amine metabolites 5-HIAA (P < 0.01) and HVA (P < 0.001) (n = 5). The concentrations of extended SP (expressed in SP equivalents) and NPK in the first 6 ml of CSF were 1.5 +/- 0.7 pM and 14.2 +/- 6.4 pM (mean +/- S.D., n = 10), respectively. The present results thus show that the levels of N-terminally extended SP and NKA are stable in frozen CSF samples for up to 5 months. The ...Continue Reading

References

Jan 1, 1992·Neuroscience and Biobehavioral Reviews·P Malek-Ahmadi
Jan 1, 1990·Life Sciences·G ToressonL Bertilsson
Sep 1, 1985·Biological Psychiatry·W H BerrettiniE S Gershon
Feb 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J E KrauseA D Hershey
Apr 30, 1985·Biochemical and Biophysical Research Communications·K TatemotoV Mutt
Jan 1, 1988·Annual Review of Neuroscience·J E Maggio
Jan 1, 1983·Peptides·M E Hall, J M Stewart

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Citations

Mar 1, 1995·Journal of Psychiatric Research·C NordinA C Zachau

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