Quantitation of nucleic acids with absorption spectroscopy

Current Protocols in Protein Science
S Gallagher

Abstract

This appendix describes a protocol for measuring absorption of nucleic acid samples at several different wavelengths to assess purity and concentration. A(260) measurements are quantitative for relatively pure nucleic acid preparations in microgram quantities. Absorbance readings cannot discriminate between DNA and RNA; however, the ratio of A at 260 and 280 nm can be used as an indicator of nucleic acid purity.

Citations

Apr 30, 2013·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·Toni A TrumboMichael Eugene Pugh
May 11, 2010·Journal of Cancer Research and Clinical Oncology·Maximo A BenavidesMaarten C Bosland
Dec 2, 2020·PloS One·Nikki L StephensonDonna M Slater

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