Quantitation of Oxylipins in Fish and Algae Oil Supplements Using Optimized Hydrolysis Procedures and Ultra-High Performance Liquid Chromatography Coupled to Tandem Mass-Spectrometry.

Journal of Agricultural and Food Chemistry
Shiva EmamiAmeer Y Taha

Abstract

Fish and algae oil supplements are enriched with eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), which are precursors to oxidized fatty acids, known as oxylipins. Here, we optimized a base hydrolysis method for measuring oxylipins in oil with ultrahigh-performance liquid chromatography coupled to tandem mass-spectrometry (UPLC-MS/MS) and quantified them in fish and algae oil supplements. Hydrolysis of 2 μL of oil with sodium carbonate resulted in greater oxylipin concentrations and minimal matrix effects, compared to higher oil volumes (10, 20, and 30 μL). Oxylipin yield was higher when oil was hydrolyzed in methanol containing 0.1% acetic acid and 0.1% butylated hydroxytoluene, compared to no methanol, and using sodium hydroxide versus sodium carbonate. Oxylipins extracted from 2 μL of oil using sodium hydroxide in solvent showed that EPA-derived oxylipins were most abundant in fish oil (84-87%), whereas DHA-oxylipins were abundant in algae oil (83%). This study shows that fish and algae oils are direct sources of EPA- and DHA-derived oxylipins.

References

May 1, 1975·Journal of Pharmaceutical Sciences·F S HomW R Ebert
Jan 25, 2000·The Journal of Biological Chemistry·M F OleksiakD C Zeldin
Apr 10, 2002·JAMA : the Journal of the American Medical Association·Frank B HuJoAnn E Manson
Mar 19, 2003·Circulation·Dariush MozaffarianUNKNOWN Cardiovascular Health Study
Mar 5, 2004·Journal of Agricultural and Food Chemistry·Yue JiangFeng Chen
Jul 22, 2004·Journal of Agricultural and Food Chemistry·Louise A Copeman, Christopher C Parrish
Feb 17, 2006·Chembiochem : a European Journal of Chemical Biology·Ulrich LionGeorg Pohnert
Jan 22, 2008·Archives of Biochemistry and Biophysics·Maude FerYolande Amet
Sep 17, 2011·Journal of Agricultural and Food Chemistry·Susan K RaatzMatthew J Picklo
May 27, 2016·Proceedings of the National Academy of Sciences of the United States of America·Derek W GilroyDavid Bishop-Bailey
Dec 25, 2016·Biochemical and Biophysical Research Communications·R Preston Mason, Samuel C R Sherratt
Feb 6, 2017·Journal of Agricultural and Food Chemistry·Christine E RichardsonAmeer Y Taha
Jul 6, 2017·Scientific Reports·Toshiharu OnoderaIichiro Shimomura
Nov 8, 2019·Prostaglandins & Other Lipid Mediators·Annika I OstermannNils Helge Schebb
Dec 7, 2019·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Fernanda Furlan Goncalves DiasJuliana Maria Leite Nobrega de Moura Bell

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.