PMID: 3766960Aug 15, 1986Paper

Quantitation of picomole levels of N-acetyl- and N-glycolylneuraminic acids by a HPLC-adaptation of the thiobarbituric acid assay

Analytical Biochemistry
L D Powell, G W Hart

Abstract

A simple HPLC adaptation of the periodate-TBA assay for free N-acetyl- and N-glycolylneuraminic acids greatly extends the sensitivity and increases the specificity of this standard colorimetric assay. The method, employing a C18 reverse-phase column eluted isocratically with a phosphoric acid-MeOH buffer, is linear over a range of 2 pmol to 20 nmol. Analyses can be performed directly on cell lysates and digests without prior purification of released sialic acids from contaminating salts and biological materials. Interference from 2-deoxysugars is completely eliminated as the chromophore from these compounds is completely resolved from that derived from sialic acids. The application of the technique to quantify cell-surface and total cellular TBA-reactive sialic acids on the surfaces of a variety of tumor cells is described. Additionally, the extent of desialylation of erythrocytes necessary to expose the T antigen is determined.

References

Jan 1, 1976·American Journal of Hematology·D AminoffN Ibgebrigtsen
May 12, 1975·Analytical Biochemistry·J Casals-StenzelR Schauer
Sep 1, 1965·The Biochemical Journal·G B Paerels, J Schut
May 1, 1973·The Histochemical Journal·R Drzeniek
Oct 22, 1968·Journal of Chromatography·D A Craven, C W Gehrke
Jan 1, 1984·Vox Sanguinis·V I Rawlinson, F Stratton

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Citations

Mar 3, 2007·Analytical and Bioanalytical Chemistry·María J MartínRicardo Rueda
Feb 1, 1992·Acta Tropica·G BardehleS Stirm
Nov 1, 1993·Molecular and Biochemical Parasitology·L C Pontes-de-CarvalhoV Nussenzweig
Feb 2, 1989·Biochimica Et Biophysica Acta·S Petrén, O Vesterberg
Nov 14, 1997·Journal of Biochemical and Biophysical Methods·E L RomeroS Alonso
Nov 1, 1996·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·A Freyberger
Feb 1, 1992·The Journal of Experimental Medicine·S SchenkmanV Nussenzweig
Mar 28, 1991·European Journal of Biochemistry·J SchallerE E Rickli
Jan 19, 2012·Nutrients·Daniel W BergnerMark S Kuhlenschmidt
Apr 5, 2016·Nature Communications·Selasi DankwaManoj T Duraisingh
Sep 8, 2006·The Journal of Biological Chemistry·Georgina N MontagnaAlberto C C Frasch
Apr 25, 2008·Current Protocols in Immunology·L D PowellH H Freeze
Aug 1, 1989·Journal of Cellular Biochemistry·G AugerA Tartar
Feb 12, 2008·Current Protocols in Molecular Biology·A E ManziA Varki
Dec 26, 2018·Critical Reviews in Analytical Chemistry·Zuzana HľasováJaroslav Katrlík
Oct 10, 1998·Journal of Virology·M D RolsmaM S Kuhlenschmidt
Nov 1, 1992·Annals of Clinical Biochemistry·P J WatersC A Pennock
Sep 16, 1988·Biochimica Et Biophysica Acta·S AfrounG Lemaire

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