PMID: 7336171Oct 1, 1981Paper

Quantitation of specific IgG antibodies in rabbits by a solid-phase radioimmunoassay with 125I-protein A from Staphylococcus aureus

Scandinavian Journal of Immunology
R LindmarkJ Sjöquist

Abstract

Radioiodinated protein A (SpA) from Staphylococcus aureus was used to quantitate specific antibodies of the IgG class at the nanogram level in rabbit serum by a standardized radioimmunoassay with the antigen covalently bound to paper discs. Quantitation of IgG in micrograms of IgG per millilitre of serum was done by using a standard curve with purified anti-bovine serum albumin (BSA) IgG or by means of the calculated molar SpA to rabbit IgG ratio of 1:1, thus converting counts per minute of 125I-SpA to nanograms of IgG. The molar ratio for SpA to human IgG was also 1:1. The unspecific binding of IgG to the paper discs was studied and could be depressed by hen serum or by adsorption of the serum sample to cellulose before the assay. The affinity constants of the extracellular SpA and human, rabbit and guinea-pig IgG, respectively, were determined and found to be close to the values previously calculated for cell-wall-bound SpA. The applicability of the assay was demonstrated by quantitation of the specific IgG response on different days after immunization of rabbits with BSA and 3,4-dinitrobenzene (DNP)-BSA.

References

Jan 1, 1978·Journal of Immunological Methods·J W Goding
Jan 1, 1979·Journal of Immunological Methods·R MarierV T Andriole
Apr 15, 1977·European Journal of Biochemistry·R LindmarkJ Sjöquist
Jan 1, 1977·Journal of Immunological Methods·J J LangoneT Borsos
Jan 1, 1976·Journal of Immunological Methods·W HeinzelH Grimminger
Jan 1, 1974·Scandinavian Journal of Immunology·G DorvalH Wigzell
Jan 1, 1974·Scandinavian Journal of Immunology·M Harboe, I Fölling
Sep 25, 1972·European Journal of Biochemistry·I BjörkJ Sjöquist
Dec 28, 1971·Biochimica Et Biophysica Acta·J I Thorell, B G Johansson

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Citations

Feb 24, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Qi ZengScott A Shippy
Jul 13, 2006·Journal of Separation Science·John E SchielDavid S Hage
Aug 12, 1983·Journal of Immunological Methods·R LindmarkJ Sjöquist
Mar 12, 1982·Journal of Immunological Methods·R LindmarkJ Sjöquist

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