Quantitation of the ABO system antigens by means of isoprecipitating antisera.

British Journal of Haematology
J PinteraE Zelená

Abstract

A quantitative method for the determination of blood group specific substances A and B on red blood cells was developed, using them to absorb isoprecipitating antisera anti-A and anti-B, followed by estimation of the residual antibody by means of a counterimmunoelectrophoretic technique with standard solutions of A and B substances prepared from saliva. The method was tested on a population of A2B donors. The mean amount of A substance in A2B RBC was 131 X 10(-12) +/- 217 X 10(-12) u/cell in the population of 95 donors. The A substance thus determined agreed significantly with values obtained by classical haemagglutinating titration. The mean amount of B substance in A2B RBC was 222 X 10(-12) +/- 178 X 10(-12) m/cell. The amounts of both the A and B substances were not correlated on the same RBC. Several peaks of the distribution curve of both substances confirmed the non-homogeneity of the populations, there being a wide standard deviation presumably due to differences in the antigen content of different subgroups.

References

Feb 1, 1973·British Journal of Haematology·J Pintera, J Vacl
Jan 1, 1970·Klinische Wochenschrift·F PesendorferF Wewalka

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Citations

Jan 1, 1978·CRC Critical Reviews in Clinical Laboratory Sciences·R C Tilton

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