PMID: 591180Oct 1, 1977

Quantitation of the beta-elimination reaction as used on glycoproteins

International Journal of Peptide and Protein Research
F DownsW Pigman


The carbohydrate side chains of mucus-type glycoproteins are O-glycosidic bonds between N-acetylgalactosamine to the hydroxyl groups of serine and threonine in the protein core. The alkaline catalyzed beta-elimination reaction, in the presence of sodium borohydride, is used for determining the number of side chains. The present paper presents a study of the quantitativeness of the alkaline borohydride procedure, using four parameters: the loss of seryl and threonyl residues, the formation of alanine and 2-aminobutanoic acid; the decrease in N-acetylhexosamine and the recovery of the amino sugar alcohols. Bovine, ovine and porcine submandibular glycoproteins were studied. Evidence is presented for the existence of N-acetylglucosamine involvement in O-glycosidic linkages to serine and threonine. Results for the relative rates of beta-elimination indicate that serine-linked glycosides are released more rapidly than threonine-linked glycosides.


Jan 1, 1977·The Biochemical Journal·A M Wu, W Pigman
Jan 1, 1977·International Journal of Peptide and Protein Research·F Downs, A Herp
Jan 1, 1976·Archives of Oral Biology·F DownsA Herp
Mar 1, 1975·Carbohydrate Research·J Moschera, W Pigman
Jan 1, 1969·International Journal of Protein Research·F Downs, W Pigman
Mar 20, 1968·Archives of Biochemistry and Biophysics·G Tettamanti, W Pigman
Jan 1, 1969·Archives of Biochemistry and Biophysics·M De Salegui, H Plonska

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