Abstract
Sulfur mustard (HD), or bis(2-chloroethyl)sulfide, has several urinary metabolites that can be measured to assess human exposure. These metabolites include the simple hydrolysis product thiodiglycol (TDG) and its oxidative analogue, TDG-sulfoxide, as well as metabolites of the glutathione/b-lyase pathway 1,1'-sulfonylbis[2-(methyl-sulfinyl)ethane] (SBMSE) and 1-methyl-sulfinyl-2-[(methylthio)ethyl-sulfonyl]ethane (MSMTESE). Current methods focus on either the TDG or the b-lyase metabolites. We have developed a single method that measures products of both metabolic branches, with the reduced compound of SBMSE and MSMTESE, 1,1'-sulfonylbis [2(methylthio)ethane] (SBMTE), as the definitive analyte and TDG as a confirmation analyte. Sample preparation included b-glucuronidase hydrolysis for TDG-glucuronide conjugates, titanium trichloride reduction of sulfoxides to SBMTE and TDG, solid-phase extraction, and a chemical derivatization. We analyzed samples using gas chromatography-tandem mass spectrometry with quantitation using isotope-dilution calibration. The method limits of detection for TDG and SBMTE were 0.5 ng/mL and 0.25 ng/mL, respectively, with relative standard deviations of less than 10%. Urine samples from individuals wit...Continue Reading
Citations
Dec 24, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ying LinJian-Wei Xie
Jun 21, 2007·Journal of Analytical Toxicology·Michael L SmithMarilyn A Huestis
Jun 14, 2014·Analytical and Bioanalytical Chemistry·Zhiyong NieJianwei Xie
Nov 24, 2011·Cutaneous and Ocular Toxicology·Amirhossein Sahebkar
Jun 30, 2009·Toxicology and Applied Pharmacology·Deep Raj SharmaKiran Dip Gill
Apr 18, 2013·Rapid Communications in Mass Spectrometry : RCM·Muntha K ReddyTimothy R Croley
Sep 26, 2015·Rapid Communications in Mass Spectrometry : RCM·Mia HalmePaula Vanninen
Nov 10, 2013·Journal of Analytical Toxicology·Tracy M AndachtRudolph C Johnson
Jan 28, 2015·Chemical Research in Toxicology·Brooke G PantazidesRudolph C Johnson
Feb 9, 2008·Antioxidants & Redox Signaling·Laszlo M HoeselPeter A Ward
Jan 27, 2005·Journal of Mass Spectrometry : JMS
Nov 4, 2005·Proteomics·Carrie L YoungJohn R Barr
Mar 19, 2019·Journal of Chromatographic Science·Rusen Koray EyisonLevent Kenar
May 11, 2019·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Sermet SezigenLevent Kenar
Jun 21, 2006·Neurotoxicity Research·C ShoneS Sharma
Sep 2, 2018·Journal of Chromatography. a·Erica ManandharBrian A Logue
Jun 23, 2020·Chemical Research in Toxicology·Sheena A Young, Benedict R Capacio