Quantitative 3-D analysis of GFAP labeled astrocytes from fluorescence confocal images

Journal of Neuroscience Methods
Prathamesh M KulkarniB Roysam

Abstract

There is a need for effective computational methods for quantifying the three-dimensional (3-D) spatial distribution, cellular arbor morphologies, and the morphological diversity of brain astrocytes to support quantitative studies of astrocytes in health, injury, and disease. Confocal fluorescence microscopy of multiplex-labeled (GFAP, DAPI) brain tissue is used to perform imaging of astrocytes in their tissue context. The proposed computational method identifies the astrocyte cell nuclei, and reconstructs their arbors using a local priority based parallel (LPP) tracing algorithm. Quantitative arbor measurements are extracted using Scorcioni's L-measure, and profiled by unsupervised harmonic co-clustering to reveal the morphological diversity. The proposed method identifies astrocyte nuclei, generates 3-D reconstructions of their arbors, and extracts quantitative arbor measurements, enabling a morphological grouping of the cell population. Our method enables comprehensive spatial and morphological profiling of astrocyte populations in brain tissue for the first time, and overcomes limitations of prior methods. Visual proofreading of the results indicate a >95% accuracy in identifying astrocyte nuclei. The arbor reconstructions ...Continue Reading

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Citations

Oct 4, 2016·Brain Structure & Function·Gabriela TavaresJoão Filipe Oliveira
Jan 8, 2021·The Journal of Comparative Neurology·Jessica BlackburnJosé Javier Otero
Aug 14, 2021·Cerebral Cortex·Eleftherios ZisisHenry Markram

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