Quantitative amino acid analysis using a Beckman system gold HPLC 126AA analyzer

Clinica Chimica Acta; International Journal of Clinical Chemistry
Y QuJ G Coldwell

Abstract

The Beckman 6300/7300 analyzer, which was widely used for amino acid (AA) analysis, is no longer commercially available. To set up an affordable AA analysis program, a Beckman system gold HPLC 126AA analyzer and Pickering Laboratories reagents were used. Two quantitative AA analysis programs were developed. One was an 18-min short program quantitating seven AAs from plasma and dried blood spots (DBS) specimens using Lithium eluents Li-365 and Li-375 at 70 degrees C column temperature. The short program could be used for diagnosis and follow-up dietary management for phenylketonuria (PKU), maple syrup urine disease (MSUD), tyrosinemia and homocystinuria patients. The second program was a 118-min long AA screening panel quantitating 40 AAs using Lithium eluents Li-275, Li-365 and Li-375 at 32, 48 and 72 degrees C column temperatures from plasma and urine specimens. The values obtained from DBS specimens were in good agreement with certified results from the Centers for Disease Control and Prevention. The values obtained from plasma and urine samples were in good correlation with those obtained from Beckman 6300 analyzer (0.9076 < or = r < or = 0.999). Amino acid quantitation from physiological samples using a Beckman 126AA Analyz...Continue Reading

References

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Citations

Feb 12, 2013·Analytical and Bioanalytical Chemistry·Chunyan WangShuying Liu
Dec 8, 2011·Journal of Pediatric Endocrinology & Metabolism : JPEM·Peng YiHuilin Niu
Feb 6, 2007·Schizophrenia Research·Uriel Heresco-LevyDaniel C Javitt
Sep 24, 2004·Biomedical Chromatography : BMC·Chiaki AoyamaKazuhiro Imai
Jun 6, 2007·Biomedical Chromatography : BMC·T Cserháti
Mar 8, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Elisabeth L SchwarzMarzia Pasquali
Sep 14, 2016·Journal of Periodontology·Martin LevineRicardo P Teles
Mar 30, 2007·Journal of Separation Science·Rania BakryGünther K Bonn
Jun 1, 2007·Electrophoresis·Susanne Kopec, Ulrike Holzgrabe
Nov 30, 2005·Electrophoresis·Christian W HuckGünther K Bonn
Apr 12, 2007·Electrophoresis·Maike SniehottaHarald Mischak
Aug 11, 2015·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Megumi KatoAkiko Takatsu

Related Concepts

Collection of Blood Specimen for Laboratory Procedure
High Pressure Liquid Chromatography Procedure
Cystathionine beta-Synthase Deficiency Disease
Optical Image Reconstruction
Maple Syrup Urine Disease, Thiamine Responsive
Hyperphenylalaninaemia
Computer Programs and Programming
Tyrosinemias

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