Quantitative analyses of GFRalpha-1 and GFRalpha-2 mRNAs and tyrosine hydroxylase protein in the nigrostriatal system reveal bilateral compensatory changes following unilateral 6-OHDA lesions in the rat

Brain Research
D A KozlowskiM C Bohn

Abstract

Copy numbers of mRNAs for GFRalpha-1 and GFRalpha-2, the preferred receptors for glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) were determined by real-time quantitative RT-PCR (QRT-PCR). Receptor expression was assessed in striatum (ST) and substantia nigra (SN) of normal rats and rats acutely or progressively lesioned by 6-OHDA injected into the medial forebrain bundle or ST, respectively. GFRalpha-1 mRNA was clearly detected in normal ST. In normal SN, significantly higher expression of both receptors was observed. At 4 weeks after acute lesion, GFRalpha-2 mRNA was markedly decreased in SN bilaterally, whereas GFRalpha-1 mRNA in SN and ST was not affected. A progressive lesion resulted in a progressive decrease of GFRalpha1 mRNA in ST bilaterally. In SN, levels of GFRalpha-1 mRNA were not significantly affected by a progressive lesion, whereas GFRalpha-2 mRNA was markedly decreased bilaterally. Quantitative western blotting standardized against tyrosine hydroxylase (TH) protein from PC12 cells revealed the expected decrease in TH protein in lesioned SN, but also significant increases in TH protein in contralateral, unlesioned SNs at 4 weeks after both acute and progressive lesions. These data suggest ...Continue Reading

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Citations

Mar 1, 2012·Journal of Neural Transmission·P Riederer, J Sian-Hülsmann
Feb 2, 2010·Neurochemical Research·Wayne A Cass, Laura E Peters
Jun 6, 2008·Neuroscience Research·Jun-Ping CaoDian-Shuai Gao
Mar 12, 2008·Prosthetics and Orthotics International·Lexyne L McNealy, Steven A Gard
Mar 30, 2010·Journal of Chemical Neuroanatomy·Phalguni Anand AlladiUday Muthane
Mar 13, 2012·Nanomedicine : Nanotechnology, Biology, and Medicine·Daniel Martinez-FongIvan Navarro-Quiroga

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