Quantitative Analyses of the Tumor Microenvironment Composition and Orientation in the Era of Precision Medicine

Frontiers in Oncology
Florent PetitprezWolf H Fridman


Tumors are formed by aggregates of cells of various origins including malignant, stromal and immune cells. The number of therapies targeting the microenvironment is increasing as the tumor microenvironment is more and more recognized as playing an essential role in tumor control. In the era of precision medicine, it is essential to precisely estimate the composition, organization and functionality of the individual patient tumor microenvironment and to find ways to therapeutically modulate it. To quantify the cell populations present in the tumor microenvironment, many tools are now available and the most recent approaches will be reviewed herein. We provide an overview of experimental and computational methodologies used to quantify tumor-associated cellular populations, including immunohistochemistry, flow and mass cytometry, bulk and single-cell transcriptomic approaches. We illustrate their respective contribution to characterize the microenvironment. We also discuss how these methods allow to guide therapeutic choices, in relation to the predictive value of some characteristics of the microenvironment.


Sep 27, 1996·International Journal of Cancer. Journal International Du Cancer·M ThurnherG Bartsch
Dec 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·B AlmandD I Gabrilovich
Oct 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aravind SubramanianJill P Mesirov
Apr 13, 2010·Nature Methods·Chatarina LarssonMats Nilsson
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
Sep 21, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Renaud Gaujoux, Cathal Seoighe
Dec 15, 2011·The Journal of Molecular Diagnostics : JMD·Fay WangYuling Luo
Mar 16, 2012·Nature Reviews. Cancer·Wolf Herman FridmanJérôme Galon
Jul 16, 2013·Nature Methods·Rongqin KeMats Nilsson
Mar 4, 2014·Nature Medicine·Michael AngeloGarry P Nolan
Mar 4, 2014·Nature Methods·Charlotte GiesenBernd Bodenmiller
Feb 18, 2015·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Nicolas A GiraldoCatherine Sautès-Fridman
Mar 31, 2015·Nature Methods·Aaron M NewmanAsh A Alizadeh
Apr 19, 2016·Immunological Reviews·Marie-Caroline Dieu-NosjeanCatherine Sautès-Fridman
Apr 24, 2016·Cancer Discovery·Rajarsi Mandal, Timothy A Chan
Jul 5, 2016·Nature Reviews. Genetics·Hubert HacklZlatko Trajanoski
Aug 16, 2016·The Journal of Clinical Investigation·Adil I DaudMichael D Rosenblum
Feb 19, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Nicolas A GiraldoCatherine Sautès-Fridman
May 6, 2017·Cell·Jonathan H ChenNir Hacohen
May 6, 2017·Cell·Stéphane ChevrierBernd Bodenmiller
Jun 22, 2017·Bioinformatics·Elias TappeinerZlatko Trajanoski
Jun 28, 2017·Nature Reviews. Clinical Oncology·Mizuki NishinoF Stephen Hodi
Jul 26, 2017·Nature Reviews. Clinical Oncology·Wolf H FridmanGuido Kroemer
Aug 5, 2017·Medical Image Analysis·Geert LitjensClara I Sánchez
Sep 9, 2017·Cancer Immunology, Immunotherapy : CII·Florent PetitprezWolf H Fridman
Oct 7, 2017·Science·Michael J T StubbingtonSarah A Teichmann
Nov 17, 2017·Genome Biology·Dvir AranAtul J Butte
Dec 2, 2017·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Janis M TaubeAshley Cimino-Mathews

❮ Previous
Next ❯


May 28, 2020·Frontiers in Immunology·Florent PetitprezWolf H Fridman
May 28, 2020·Cancers·Stefano FregaLaura Evangelista
Sep 15, 2020·Frontiers in Molecular Biosciences·Anca F SavulescuMusa M Mhlanga
Jul 22, 2019·Cancer Letters·Rebeca Burgos-PanaderoRosa Noguera
Oct 5, 2020·Seminars in Immunology·Johanna VerneauCheng-Ming Sun

❮ Previous
Next ❯

Methods Mentioned

flow cytometry

Software Mentioned

Gene Set Enrichment Analysis [ GSEA

Related Concepts

Related Feeds

Cancer -Omics

A variety of different high-throughput technologies can be used to identify the complete catalog of changes that characterize the molecular profile of cohorts of tumor samples. Discover the latest insights gained from cancer 'omics' in this feed.