Quantitative analysis and characterization of biofunctionalized fluorescent silica particles

Langmuir : the ACS Journal of Surfaces and Colloids
Simon R CorrieMatt Trau

Abstract

A strategy for the production and subsequent characterization of biofunctionalized silica particles is presented. The particles were engineered to produce a bifunctional material capable of both (a) the attachment of fluorescent dyes for particle encoding and (b) the sequential modification of the surface of the particles to couple oligonucleotide probes. A combination of microscopic and analytical methods is implemented to demonstrate that modification of the particles with 3-aminopropyl trimethoxysilane results in an even distribution of amine groups across the particle surface. Evidence is provided to indicate that there are negligible interactions between the bound fluorescent dyes and the attached biomolecules. A unique approach was adopted to provide direct quantification of the oligonucleotide probe loading on the particle surface through X-ray photoelectron spectroscopy, a technique which may have a major impact for current researchers and users of bead-based technologies. A simple hybridization assay showing high sequence specificity is included to demonstrate the applicability of these particles to DNA screening.

References

Aug 5, 2000·Il Farmaco·W T MarkiewiczM Markiewicz
Mar 14, 2001·Journal of Biochemical and Biophysical Methods·M K WalshB C Weimer
Dec 18, 2001·Trends in Biotechnology·John P Nolan, Larry A Sklar
Mar 22, 2002·Trends in Biotechnology·Bronwyn J Battersby, Matt Trau
Aug 31, 2002·Science·Uri Raviv, Jacob Klein
Dec 20, 2003·Combinatorial Chemistry & High Throughput Screening·Boon-ek YingyongnarongkulMark Bradley
Mar 30, 2004·Biopolymers·Gali SteinbergChanfeng Zhao
Oct 7, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Chris R MillerMatt Trau

❮ Previous
Next ❯

Citations

Aug 2, 2007·Chemical Communications : Chem Comm·Brian G TrewynVictor S-Y Lin
Jul 31, 2008·Physical Chemistry Chemical Physics : PCCP·Zhong-Jie Jiang, Werner A Goedel
Oct 20, 2009·Journal of Biotechnology·Ashley R ConnollyMatt Trau
Jan 13, 2009·Journal of Colloid and Interface Science·Hirokazu MiyoshiHirotaro Mori
Mar 15, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·S R CorrieM Trau
Mar 29, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Dominik SarmaKnut Rurack
Jul 1, 2015·Chemical Communications : Chem Comm·Lily Nari KimSunghoon Kwon
Nov 10, 2012·Journal of Colloid and Interface Science·Wenshi MaHong Wang
Oct 22, 2016·New Biotechnology·Elena Pérez-RuizDragana Spasic
Mar 25, 2010·Bioconjugate Chemistry·Ramkumar PalanisamyMatt Trau
Dec 8, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Chih-Hsin Chen, Kun-Lin Yang
Oct 1, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Robert VogelHalina Rubinsztein-Dunlop
Oct 27, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Meiyun XuChangmin Yu
Feb 25, 2017·Biomacromolecules·Ngozi A EzeValeria T Milam
May 29, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Julia A WalkerSimon R Corrie

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.