Quantitative analysis of amitriptyline and nortriptyline in human plasma and liver microsomal preparations by high-performance liquid chromatography

Journal of Chromatography. B, Biomedical Applications
P Ghahramani, M S Lennard

Abstract

A simple, rapid, highly selective and sensitive method for the analysis of amitriptyline and nortriptyline in plasma and human liver microsomes is described. It is suitable for the routine analysis of large numbers of samples using readily available instrumentation and low cost consumables. The detection limit was 2 ng/ml for both compounds and calibration curves were linear over a wide range of concentrations and passed through the origin. The within-batch and between-batch coefficients of variation for amitriptyline and nortriptyline were less than 7.4% and 12.8%, respectively. A series of compounds, including inhibitors used for probing cytochrome P450 activity in vitro, were tested for interference in the assay. Only ketoconazole caused interference and the assay was modified to allow samples containing ketoconazole to be analysed.

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Citations

Aug 26, 2003·Archives of Pharmacal Research·Eun-Seok ParkSang-Cheol Chi
Oct 13, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Salah Khanahmadzadeh, Ahmad Tarigh
Jan 31, 2002·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yoshihiro SaitoKiyokatsu Jinno
Jun 9, 2009·International Journal of Pharmaceutics·A MeleroU F Schaefer
Apr 3, 2008·The Journal of Pharmacy and Pharmacology·Virginia MerinoMatilde Merino-Sanjuán
Jul 28, 2005·Journal of Pharmacological and Toxicological Methods·Devasish BoseMayte Gil-Agustí
Feb 10, 2010·Pharmaceutical Development and Technology·José Juan Escobar-ChávezMatilde Merino-Sanjuán
Jun 2, 2009·Journal of Forensic and Legal Medicine·Ioannis StiakakisAristidis M Tsatsakis
Jun 17, 2006·Therapeutic Drug Monitoring·Soo-Youn LeeJong-Won Kim
Feb 23, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Patricia AlmudeverManuel Alós

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