Quantitative analysis of antibiotics by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Rapid Communications in Mass Spectrometry : RCM
Y C LingY T Chen

Abstract

Comparative studies of the matrix-assisted laser desorption/ionization (MALDI) of 30 antibiotics were made using alpha-cyano-4-hydroxycinnamic acid (HCCA), 2,5-dihydroxybenzoic acid (DHB), 5,10,15,20-tetrakis(4-hydroxyphenyl)-21H,23H-porphyrin and meso-tetra(N-methyl-4-pyridyl)porphyrin matrices. Most antibiotics generated intense protonated molecules in HCCA and DHB matrices, and sodium or potassium adduct ions in porphyrin matrices. Using sulfonamide antibiotics as model compounds, DHB was found to be the most suitable matrix for quantitative analysis. Detection limits were about 1 picomole. Linear correlation (R2 > 0.97), between the sample quantity over the range 1 to 100 picomole and the signal response, was obtained when ratios of the sum of peak areas of protonated molecules and sodium adduct ions, with reference to that of a structurally analogous internal standard (acetaminophen), were measured. The precision was found to be in the range of 4 to 32% relative standard deviation, dependent on the type and concentration of the analyte. A simple acylation derivatization process was developed to confirm the presence of suspected antibiotic residues. It is demonstrated that MALDI is a viable technique for the quantitative an...Continue Reading

Citations

Apr 29, 2010·Chemical Reviews·Kamila Chughtai, Ron M A Heeren
Sep 21, 2010·Journal of Pharmaceutical and Biomedical Analysis·Jan MatysiakZenon J Kokot
Mar 12, 2004·Biochemical and Biophysical Research Communications·Jeroen J A van KampenTheo M Luider
Oct 15, 2003·Rapid Communications in Mass Spectrometry : RCM·Panos HatsisDietrich A Volmer
Mar 17, 2006·Rapid Communications in Mass Spectrometry : RCM·Seetharaman VaidyanathanRoyston Goodacre
Apr 22, 2006·Rapid Communications in Mass Spectrometry : RCM·Jon G WilkesAlexandre A Shvartsburg
Jun 5, 2007·Rapid Communications in Mass Spectrometry : RCM·Seetharaman Vaidyanathan, Royston Goodacre
Mar 15, 2003·Analytical and Bioanalytical Chemistry·DebraLynn Mims, David Hercules
Apr 9, 2008·Analytical Chemistry·Jeroen J A van KampenTheo M Luider
Jun 12, 2002·Journal of the American Society for Mass Spectrometry·Matthew C CrowePaul Hergenrother
Sep 27, 2002·Journal of the American Society for Mass Spectrometry·Martin BucknallMark W Duncan

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