Quantitative analysis of Caenorhabditis elegans chemotaxis using a microfluidic device

Analytica Chimica Acta
Liang HuBi-Feng Liu

Abstract

Caenorhabditis elegans, one of the widely studied model organisms, sense external chemical cues and perform relative chemotaxis behaviors through its simple chemosensory neuronal system. To study the mechanism underlying chemosensory behavior, a rapid and reliable method for quantitatively analyzing the worms' behaviors is essential. In this work, we demonstrated a microfluidic approach for investigating chemotaxis responses of worms to chemical gradients. The flow-based microfluidic chip was consisted of circular tree-like microchannels, which was able to generate eight flow streams containing stepwise chemical concentrations without the difference in flow velocity. Worms' upstream swimming into microchannels with various concentrations was monitored for quantitative analysis of the chemotaxis behavior. By using this microfluidic chip, the attractive and repellent responses of C. elegans to NaCl were successfully quantified within several minutes. The results demonstrated the wild type-like repellent responses and severely impaired attractive responses in grk-2 mutant animals with defects in calcium influx. In addition, the chemotaxis analysis of the third stage larvae revealed that its gustatory response was different from th...Continue Reading

References

Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·S Ward
Jan 29, 1999·Nature·U AlonS Leibler
Aug 30, 2002·Analytical Chemistry·Virginie MengeaudHubert H Girault
Apr 22, 2003·Proceedings of the National Academy of Sciences of the United States of America·Hanbin MaoMichael D Manson
Aug 13, 2004·Journal of Computational Neuroscience·Nathan A DunnJohn S Conery
Jan 13, 2006·The EMBO Journal·Renate K HukemaGert Jansen
Jul 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Robert Qing MiaoWilliam C Sessa
Feb 20, 2007·Annual Review of Physiology·Richard T Premont, Raul R Gainetdinov
Jun 1, 2007·Lab on a Chip·Shing-Yi ChengMingming Wu
Nov 22, 2007·Lab on a Chip·Nannan YeBingcheng Lin
Dec 1, 2007·WormBook : the Online Review of C. Elegans Biology·Cornelia I Bargmann
Feb 29, 2008·Biotechnology and Bioengineering·Larry M LanningTao Long
Dec 17, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jonathan T Pierce-ShimomuraSteven L McIntire
May 5, 2009·Applied and Environmental Microbiology·Derek L EnglertArul Jayaraman
Mar 26, 2010·Learning & Memory·Evan L Ardiel, Catharine H Rankin
Sep 3, 2010·Biosensors & Bioelectronics·Heon-Ho JeongChang-Soo Lee
Oct 16, 2010·Integrative Biology : Quantitative Biosciences From Nano to Macro·Dawit JowharChristopher Janetopoulos
Oct 23, 2010·Integrative Biology : Quantitative Biosciences From Nano to Macro·Tanvir AhmedRoman Stocker
Apr 26, 2011·Topics in Current Chemistry·Weiwei ShiJianhua Qin
Dec 1, 1998·Analytical Chemistry·D C DuffyG M Whitesides
Oct 1, 2011·The Journal of Physical Chemistry. B·Tommaso CasaliniCarlo Cavallotti
Mar 14, 2012·Proceedings of the National Academy of Sciences of the United States of America· MarcosRoman Stocker

❮ Previous
Next ❯

Citations

Aug 5, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Muniesh Muthaiyan Shanmugam, Tuhin Subhra Santra
Mar 12, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Xiaowen OuBi-Feng Liu
Dec 12, 2017·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Sunhee YoonSun Min Kim
Jul 19, 2016·Micromachines·Bhagwati P Gupta, Pouya Rezai
Oct 3, 2018·PLoS Neglected Tropical Diseases·Shunyu LiSung-Jong Hong
Mar 15, 2019·Biomicrofluidics·Naoki Yanagisawa, Tetsuya Higashiyama

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.