Quantitative analysis of carbonic anhydrase IX mRNA in human non-small cell lung cancer

Lung Cancer : Journal of the International Association for the Study of Lung Cancer
Lisa SimiClaudio Orlando

Abstract

Hypoxia is associated with malignant progression and poor outcome in human cancers. The effects of hypoxia are mediated by a series of genomic changes that enable tumor cells to survive or escape their oxygen deficient environment. Recent studies indicated that carbonic anhydrase IX (CA IX) is an intrinsic marker of hypoxia. In the present study we investigated with quantitative RT-PCR the expression of CA IX mRNA in 93 non-small cell lung carcinomas (NSCLC) and in their paired not affected tissues. CA IX mRNA was expressed in 100% NSCLC and in 76% of paired not affected tissues, even if tumoral CA IX expression was found constantly higher (p < 0.02) than that found in normal tissues. The increase of CA IX mRNA expression in cancer tissues was significantly correlated to the increase of corresponding protein, as determined with conventional immunoblotting (p = 0.027). In addition the expression of CA IX mRNA in NSCLC samples was significantly correlated to VEGF (p = 0.002) and MMP-9 (p = 0.002) mRNAs. Whereas CA IX mRNA expression was not associated to any clinical-pathological parameters in our patients, global survival analysis of cancer-related death revealed that high expression of CA IX mRNA predicted unfavorable outcome (...Continue Reading

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Citations

Jun 19, 2007·Antioxidants & Redox Signaling·Eui Jung MoonMark W Dewhirst
Feb 28, 2009·BMC Bioinformatics·Md Rafiul HassanKotagiri Ramamohanarao
Feb 1, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Daniel E B SwinsonKenneth J O'Byrne
Mar 26, 2014·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·David J StewartIgnacio Ivan Wistuba
Aug 6, 2010·Seminars in Radiation Oncology·Edward E GravesQuynh-Thu Le
Feb 6, 2009·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Elisabeth KodymDebabrata Saha
Nov 22, 2008·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Francesca MalentacchiClaudio Orlando
Jun 15, 2007·Microvascular Research·Koami DagnonPatrice Callard
Aug 1, 2015·Pathology Oncology Research : POR·Yuji LiLongping Huang
Oct 13, 2006·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Hidehiro Kon-noAtsushi Ochiai
Aug 6, 2013·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Marius IliePaul Hofman
Feb 26, 2009·British Journal of Cancer·E KorkeilaS Pyrhönen
Jan 11, 2018·Scientific Reports·Victor D MartinezKevin L Bennewith
Jan 27, 2018·Oncotarget·Allison S CohenDavid L Morse

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