Quantitative analysis of catalysis and inhibition at horseradish peroxidase monolayers immobilized on an electrode surface

Journal of the American Chemical Society
Benoît LimogesDounia Yazidi

Abstract

Out of several tries, biotinylation of the electrode surface by means of a sacrificial biotinylated immunoglobulin, followed by the anchoring of an avidin-enzyme conjugate appears as the best procedure for depositing a horseradish peroxidase (HRP) monolayer onto an electrode surface, allowing a high-yield immobilization of the enzyme within a stable and highly catalytic coating. Cyclic voltammetry is an efficient means for analyzing the catalytic reduction of H(2)O(2) at such HRP monolayer electrodes in the presence of [Os(III)(bpy)(2)pyCl](2+) (with bpy = bipyridine and py = pyridine) as a one-electron reversible cosubstrate. The odd shapes of current-potential responses, unusual bell-shaped variation of the peak or plateau current with the substrate concentration, hysteresis and trace crossing phenomena, and dependence or lack of dependence with the scan rate, can all be explained and quantitatively analyzed in the framework of the same catalysis/inhibition mechanism as previously demonstrated for homogeneous systems, taking substrate and cosubstrate mass transport of into account. According to H(2)O(2) concentration, limiting-behavior analyses based on the dominant factors or complete numerical simulation were used in the tr...Continue Reading

References

Apr 1, 1992·Enzyme and Microbial Technology·J P O'DalyR W Henkens
Oct 18, 1990·Biochimica Et Biophysica Acta·M B ArnaoF García-Cánovas
Nov 15, 1990·Analytical Chemistry·M H Smit, A E Cass
Apr 15, 1995·Analytical Chemistry·T de Lumley-WoodyearA Heller
Apr 17, 1999·Trends in Biotechnology·S ColonnaP Pasta
Oct 3, 1999·Biotechnology and Bioengineering·S V RaoL G Bachas
Jan 10, 2002·Journal of the American Chemical Society·Murielle DequaireJean-Michel Savéant
Jan 25, 2002·Analytical Chemistry·Charles N CampbellAdam Heller
Apr 19, 2002·Journal of the American Chemical Society·Kevyn SmithRobert M Parker
Sep 19, 2002·Analytical Chemistry·Murielle Dequaire, Adam Heller
Nov 20, 2002·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Yen WeiQiuwei Feng

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Citations

Dec 7, 2007·The Journal of Physical Chemistry. B·Haiyun LuNaifei Hu
Apr 13, 2006·Chemical Communications : Chem Comm·Sakandar RaufDae-Joon Kang
Aug 16, 2005·Biosensors & Bioelectronics·E ZaccoS Alegret
Jan 16, 2007·Analytical Biochemistry·Graciela PrianoFernando Battaglini
Sep 4, 2007·Journal of Inorganic Biochemistry·Aditi Das, Michael H Hecht
Feb 24, 2006·The Analyst·Seong Jung KwonJuhyoun Kwak
May 31, 2018·Physical Chemistry Chemical Physics : PCCP·Lifu ChenRichard G Compton
Nov 16, 2010·Chemistry : a European Journal·Diego PallarolaFernando Battaglini
Apr 28, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Bertrand ReuillardSerge Cosnier
Oct 2, 2012·Chemical Communications : Chem Comm·M Lorena CortezFernando Battaglini
May 21, 2008·Journal of the American Chemical Society·Benoît LimogesBernd Schöllhorn
Apr 11, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Paula PescadorEdwin Donath
Nov 30, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Yasufumi TakahashiTomokazu Matsue
Nov 30, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Claude P AndrieuxDounia Yazidi
Aug 11, 2010·The Journal of Physical Chemistry. B·Alexander K BuellChristopher M Dobson
Aug 2, 2005·Analytical Chemistry·Graciela Priano, Fernando Battaglini
Sep 30, 2005·Journal of the American Chemical Society·Gabriele DiekertJean-Michel Savéant
Jan 12, 2007·The Journal of Physical Chemistry. B·Rafael AndreuJuan Jose Calvente

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