Quantitative analysis of clonal bone marrow CD19+ B cells: use of B cell lineage trees to delineate their role in the pathogenesis of light chain amyloidosis

Clinical Immunology : the Official Journal of the Clinical Immunology Society
Michelle K ManskeRoshini S Abraham

Abstract

Light chain amyloidosis (AL) is a bone marrow (BM) plasma cell neoplasia with systemic deposition of Ig light chain amyloid fibrils. Here, we report the identification of clonal CD19 B cells in the BM and the use of a novel mathematical algorithm to generate B cell lineage trees of the clonal CD19 B cells and CD138 plasma cells from the BM of AL patients to delineate the relationship between these two clonal populations. The CD19+ clonal B cells in the BM of AL patients related to the clonal plasma cells represent a pre-plasma cell precursor population. The B cell lineage trees from AL patients also show significant differences in clonal diversification and antigenic selection compared to clones from normal, healthy controls. These data provide a robust example of the use of graphical quantification methods in delineating the role of neoplastic precursors in the pathogenesis of hematopoietic malignancies.

References

Oct 1, 1977·The Journal of Clinical Investigation·A Hamburger, S E Salmon
Dec 1, 1989·Mayo Clinic Proceedings·M A Gertz, R A Kyle
Sep 1, 1985·The Journal of Clinical Investigation·F Caligaris-CappioG Janossy
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C Kocks, K Rajewsky
Aug 2, 1987·Nature·M J ShlomchikM G Weigert
Jun 1, 1997·Molecular Biology and Evolution·T B Kepler
Dec 12, 1997·European Journal of Immunology·D K Dunn-WaltersJ Spencer
Mar 20, 2002·The Journal of Experimental Medicine·Brian P O'ConnorRandolph J Noelle
Oct 31, 2002·International Immunology·Kara M JohnsonPamela L Witte
Nov 25, 2003·Blood·William MatsuiRichard J Jones
Nov 18, 2005·International Immunology·Gitit ShahafRamit Mehr

❮ Previous
Next ❯

Citations

Apr 4, 2007·Immunology and Cell Biology·Ravit SaadaRamit Mehr
Sep 11, 2009·Nucleic Acids Research·Michal BarakRamit Mehr
Sep 30, 2010·Immunome Research·Zhiliang ChenBruno A Gaëta
Oct 20, 2018·Frontiers in Immunology·Alexander Dimitri YermanosSai T Reddy

❮ Previous
Next ❯

Related Concepts

Related Feeds

B cell Differentiation

Depending on the signal received through the B cell receptor and other receptors, B cells differentiate into follicular or marginal zone B cells. Here is the latest research pertaining to this differentiation process.