Quantitative analysis of Fusarium mycotoxins in maize using accelerated solvent extraction before liquid chromatography/atmospheric pressure chemical ionization tandem mass spectrometry

Food Additives and Contaminants
D RoyerP A Guy

Abstract

A method for the simultaneous quantitative determination of deoxynivalenol (DON), fumonisin B1 (FB1) and zearalenone (ZEN) in maize by liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometry (LC-APCIMS/MS), using stable isotopically labelled and structural analogues internal standards, is described. The procedure involves accelerated solvent extraction followed by two solid-phase clean-up steps on strong anion exchange resin and a Mycosep column. Typical recoveries were calculated by spiking blank maize at three different concentrations for deoxynivalenol (200, 400 and 1000 microg kg(-1)) at 70%, for fumonisin B1 (100, 200 and 1000 microg kg(-1)) at 90%, and for zearalenone (50, 100 and 200 microg kg(-1)) at 40%. LC-APCIMS/MS analyses were realized in collision-induced dissociation on an ion-trap instrument to provide a high degree of selectivity and sensitivity. Extraction of ions from two transition reactions, monitored by LC-APCIMS/MS for each analyte, enabled a limit of detection for DON, FB1 and ZEN at, respectively, 10, 20 and 3 microg kg(-1), and a limit of quantification at, respectively, 50, 50 and 10 microg kg(-1). The robustness of the method was also evaluated with the analysis of whe...Continue Reading

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Citations

Apr 19, 2011·Journal of Agricultural and Food Chemistry·Emmanuel Njumbe EdiageSarah De Saeger
Oct 25, 2008·Chemical Society Reviews·Gordon Seymour Shephard
Jul 16, 2005·Food Additives and Contaminants·A BiancardiR Marchelli
Feb 21, 2008·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Rudolf KrskaColin Crews
Aug 15, 2009·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Jose Diana Di MavunguSarah De Saeger
Jul 11, 2013·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Marcin BryłaJanusz Sękul
Apr 1, 2009·International Journal of Molecular Sciences·Irena Kralj Cigić, Helena Prosen
Nov 19, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xiaoqin CaoHong Ding
Sep 1, 2007·International Journal of Food Microbiology·Franz BerthillerRainer Schuhmacher
Apr 7, 2007·International Journal of Food Microbiology·Timothy O AdejumoPetr Karlovsky
Apr 4, 2015·Critical Reviews in Food Science and Nutrition·Lijuan XieYibin Ying
Sep 1, 2005·Journal of Chromatography. a·R Carabias-MartínezJ Hernández-Méndez
Jan 24, 2013·Journal of Separation Science·Marcin BryłaMałgorzata Rzepkowska
Dec 4, 2008·Rapid Communications in Mass Spectrometry : RCM·Sofie MonbaliuSarah De Saeger
Oct 18, 2005·Molecular Nutrition & Food Research·Michael BretzHans-Ulrich Humpf
Mar 8, 2006·Molecular Nutrition & Food Research·Michael BretzHans-Ulrich Humpf
Jul 18, 2020·Journal of Food Protection·Shahzad Zafar IqbalNik Iskandar Samsudin
Jan 27, 2005·Journal of Mass Spectrometry : JMS
Sep 21, 2007·Journal of Agricultural and Food Chemistry·Benedikt CramerHans-Ulrich Humpf

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