Quantitative analysis of gap-junctional intercellular communication in precision-cut mouse liver slices

Cell and Tissue Research
Alessandro RomualdiT Ott

Abstract

Direct intercellular communication through gap junction channels is involved in the maintenance of tissue homeostasis and suppression of carcinogenesis. Gap-junctional communication is often altered in tumor cells but it can also be modulated in response to tumor promotors or inflammatory signals. In order to evaluate the effect of nongenotoxic compounds, suggested to be involved in tumor promotion, on gap junctional intercellular communication in the liver, we have developed a direct dye transfer method. The fluorescent dye Alexa Fluor 488 was iontophoretically injected into hepatocytes of freshly prepared, precision-cut mouse liver slices (250 microm). The area of dye spreading was monitored and quantified by microscopy. Comparison of dye spreading in connexin-32-deficient versus wild-type liver revealed a 96% decrease in connexin-32-deficient tissue. Induction of an acute phase response in connexin-32-deficient mice by intraperitoneal injection of lipopolysaccharide increased dye coupling by 33%, probably due to upregulation of connexin-26-containing gap junction channels.

Citations

Jun 14, 2003·Proceedings of the National Academy of Sciences of the United States of America·Bertrand LeobonSerge Charpak
Jul 29, 2009·Critical Reviews in Biochemistry and Molecular Biology·Mathieu VinkenVera Rogiers
Sep 1, 2004·Biochemical and Biophysical Research Communications·Paulo R A V CorreaMichael H Nathanson
Dec 20, 2005·Biochimica Et Biophysica Acta·Marc MesnilMaria-Lucia Zaidan-Dagli
Jul 25, 2003·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Dagmar NeupertDieter Müller
Oct 7, 2004·Journal of Cell Science·Julia von MaltzahnGoran Söhl
Jul 4, 2003·Journal of Cell Science·Markus KretzThomas Ott

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