Quantitative Analysis of Glucose Metabolic Cleavage in Glucose Transporters Overexpressed Cancer Cells by Target-Specific Fluorescent Gold Nanoclusters

Analytical Chemistry
Tsai-Mu ChengChia-Chun Chen

Abstract

The glucose metabolism rate in cancer cells is a crucial piece of information for the cancer aggressiveness. A feasible method to monitor processes of oncogenic mutations has been demonstrated in this work. The fluorescent gold nanoclusters conjugated with glucose (glucose-AuNCs) were successfully synthesized as a cancer-targeting probe for glucose transporters (Gluts) overexpressed by U-87 MG cancer cells, which can be observed under confocal microscopy. The structural and optical characterizations of fluorescent glucose-AuNCs were confirmed by transmission electron microscope (TEM) and Fourier transform infrared spectroscopy (FTIR). The MTT assay exhibited the high biocompatibility of water-soluble glucose-AuNCs for further biomedical applications. The glucose metabolic cleavage of glucose-AuNCs by glycolytic enzymes from U-87 MG cancer cell was measured by fluorescence change of glucose-AuNCs. The fluorescence change based on the integrated area under fluorescence spectra ( A t) of glucose-AuNCs was plotted as a function of different reaction time ( t) with glycolytic enzymes. The fitted curve of A t versus t showed the first-order kinetics to explain the mechanism of glucose metabolic cleavage rate of glucose-AuNCs by glyco...Continue Reading

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Citations

May 3, 2019·Journal of Biomaterials Science. Polymer Edition·Qianqian Guo, Xinge Zhang
Jun 19, 2019·International Journal of Molecular Sciences·Sibidou YougbareTsung-Rong Kuo
Jul 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Ganeshlenin Kandasamy, Dipak Maity
Aug 15, 2019·ACS Applied Materials & Interfaces·Quan-Quan ZhuangWei Chen
Nov 28, 2021·Nanomaterials·Shih-Hua TanTsung-Rong Kuo

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