PMID: 8971581Sep 1, 1996Paper

Quantitative analysis of heparin retention on heparin bonded knitted Dacron grafts after exposure to shear stress in vitro

International Angiology : a Journal of the International Union of Angiology
P Swartbol, L Norgren

Abstract

To analyse the quantity of heparin retention on heparin bonded collagen coated knitted Dacron grafts (HCD) and heparin bonded knitted Dacron grafts without interluminal collagen coating (HD) after they had been exposed to shear stress in an artificial circulation model. A comparative study. University Hospital, Department of Surgery. HCD grafts were exposed to shear stress during 2, 24, 48, and 168 hours, and HD grafts during 1, 2, 3 and 4 weeks. Heparin retention on the graft walls was determined by a method based on the measurements of total sugars. HCD grafts showed no loss of heparin from the graft-surface when exposed to a flow of 200 ml/min up to 168 hrs. HD grafts demonstrated an initial loss of heparin from 8 mg/g to 3 mg/g/graft-segment within one week after which the level was stable during the remaining three weeks. Based on the present experimental setting it seems reasonable to conclude that the heparin-collagen layer remains stable but heparin alone is partly washed out by shear stress. To measure the release of heparin under the influence of the bloodstream including mechanisms for the slow degradation of collagen, a more sensitive method for heparin analysis is required.

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.